The Effects of Interleukin 2, Gamma Interferon, and B Cell Differentiation Factor on the Differentiation of Human B Cells
It has been well known that in the human system, SAC-activated B cells proliferate and differentiate in response to helper T cell factors. Among many factors included within the broad category of helper T cell factors, three distinct factors (interleukin 2 IL-2, gamma interferon γ-IFN, and B cell differentiation factor BCDF) were reported to be involved in B cell differentiation. Recent progress in recombinant DNA technology has made highly purified IL-2 and γ-IFN available, and even though BCDF is not yet cloned by molecular biological techniques, two kinds of BCDFs derived from the supernatants of newly established cell lines have been recently reported. In the present study, employing normal B cells or EBV-transformed B cell lines, we have investigated the effects of these factors on human B cells.
KeywordsEpic Versus Molecular Biological Technique Plaque Form Cell Percoll Gradient Centrifugation Cell Differentiation Factor
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