Transcriptional and Post-Transcriptional Control of Ig-Gene Expression in Murine B-Cells Activated by LPS and Anti-Receptor Antibodies

  • Una Chen-Bettecken
  • Eberhard Wecker
  • Anneliese Schimpl
Part of the NATO ASI Series book series (NSSA, volume 123)


Bacterial Lipopdysaccharide (LPS) induces normal resting B-cells to proliferate and to differentiate into immunoglobulin (Ig)-secreting plasma cells. We have studied the transcriptional control of Ig-gene expression in this system by an in vitro transcriptional run-on assay (1). Fig. 1 shows the relative RNA transcriptional rates in nuclei isolated from resting B-cells and from B-cells 1 to 4 days after LPS stimulation. There are very few if any transcripts demonstrable in resting B-cells with the probes tested (C/u, Igh-enhancer, and kappa). Even actin and H-2 probes do not give strong signals. Upon LPS stimulation, there is a rapid and strong enhancement of RNA Polymerase II activities until day 4, giving 30–50 fold increases. The /u- and kappa-transcripts detectable are about equal, indicating also a balanced distribution of RNA Polymerase II along both heavy and light chain loci. The 30–50 fold increases of transcription upon LPS induction account for the strong accumulation of Ig-mRNAs found in day-4 LPS cultures. The data demonstrate that Ig-gene expression in normal activated B-lymphocytes is regulated primarily at the level of transcription.


Transcriptional Rate Light Chain Locus Kappa locuS Initial Inhibitory Effect Lysate Translation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Mather, E.L., Nelson, K.J., Haimovich, J. and Perry, R.P. (1984). Cell 36, 329–338.CrossRefGoogle Scholar
  2. 2.
    Parker, D.C. (1980). Immunol. Rev. 52, 115.CrossRefGoogle Scholar
  3. 3.
    Chen-Bettecken, U., Wecker, E. and Schimpl, A. (1985). Proc. Natl. Acad. Sci. USA November.Google Scholar
  4. 4.
    Andersson, J., Bullock, W.W. and Melcher, F. (1974). Eur. J. Immunol 4, 715–722.CrossRefGoogle Scholar
  5. 5.
    Webb, C.F., Gatings, W.E. and Cooper, M.D. (1983). Eur. J. Immunol. 13, 556–559.CrossRefGoogle Scholar
  6. 6.
    Pelhem, H.R.B. and Jackson, R.J. (1976). Eur. J. Biochem. 67, 247–256.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Una Chen-Bettecken
    • 1
  • Eberhard Wecker
    • 1
  • Anneliese Schimpl
    • 1
  1. 1.Institut für Virologie und ImmunbiologieUniversität WürzburgWurzbürgGermany

Personalised recommendations