Proliferation and Idiotype Secretion by Human B-Cell Non-Hodgkin’s Lymphoma Cells in vitro
The neoplastic cells in human B-cell non-Hodgkin’s lymphoma (B-NHL) can be regarded as monoclonal expansions of lymphocytes arrested in a particular stage of the normal B-cell differentiation pathway. However, in recent years it has become evident that this arrest is not absolute and that neoplastic B cells can be induced to mature further into immunoglobulin (Ig) secreting cells in vitro if provided with B-cell growth and/or differentiation factors (BCGF, BCDF), which operate in normal B-cell maturation (reviewed in ref. 1). Recently, interleukin-2 (IL-2) has been found to exert proliferative and differentiation inducing activity towards normal and leukemic B cells as well (2,3,4). We have analysed the functional capabilities of human B-NHL cells upon culture in serumfree medium without any deliberate addition of growth or differentiation inducing factors. Proliferation and Ig secretion were found to be related to phenotypic marker expression, especially of activation-associated antigens. These parameters were compared with Idiotype+ (Id+) Ig secretion in vivo.
KeywordsDifferentiation Induce Factor Phenotypic Marker Expression Serumfree Medium Differentiation Induce Activity
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