A Model for the First Activation Cycle of Human B Lymphocytes
The human B lymphocyte provides an excellent model for examining cell cycle control and its undermining by malignant transformation. Resting B cells can be isolated in both high yield and purity and their response to a variety of defined stimuli followed in detail. Of particular interest is the activator Epstein-Barr virus (EBV) which infects human B cells via their receptors for the C3d fragment of complement (CR2) and subsequently bestows immortality through expression of the viral genome1. P3HR-1, a mutant strain of EBV, while retaining CR2-binding capacity, lacks a deletion in the EBNA2 transforming region and fails to induce growth in infected cells2. In this report we detail the outcome of exposing resting B cells to both the transforming and non-transforming strains of EBV within the context of the mitotic cell cycle and compare the results with those obtained from activators which use more physiological routes of B-cell triggering.
KeywordsAcridine Orange Inositol Phosphate Inositol Trisphosphate Mitotic Cell Cycle Inositol Phospholipid
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