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Regulation of the Assembly and Expression of Immunoglobulin Genes: Variable Region Assembly and Heavy Chain Class Switching

  • R. A. DePinho
  • G. D. Yancopoulos
  • T. K. Blackwell
  • M. G. Reth
  • K. Kruger
  • S. G. Lutzker
  • F. W. Alt
Part of the NATO ASI Series book series (NSSA, volume 123)

Abstract

To elucidate further the general mechanisms which control the assembly and expression of Immunoglobulin variable region gene segments and heavy-chain class switching, we have exploited Abelson murine leukemia virus (A-MuLV) transformed pre-B cells as a model system. The Abelson virus is a replication defective retrovirus which is unique in its capacity to transform very early B-lineage cells in vitro (1). A-MuLV transformants generated from murine fetal liver or adult bone marrow have provided both a static representation of various pre-B cell differentiation stages, as well as a dynamic view of pre-B cell immunodifferentiation events (2,3). Our analyses of these lines have provided a variety of new and sometimes surprising findings regarding the early stages of B cell differentiation; and importantly, all of these novel aspects first observed in A-MuLV transformants were found to reflect events that occur early in normal B lineage cells in vitro. Furthermore, we have utilized gene transfer technology to gain additional insight into the molecular events involved with the regulation of immunogloblin and T cell receptor variable region (V) gene assembly (3).

Keywords

Gene Segment Class Switch Immunoglobulin Heavy Chain Variable Region Gene Allelic Exclusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • R. A. DePinho
    • 1
  • G. D. Yancopoulos
    • 1
  • T. K. Blackwell
    • 1
  • M. G. Reth
    • 1
  • K. Kruger
    • 1
  • S. G. Lutzker
    • 1
  • F. W. Alt
    • 1
  1. 1.College of Physicians and Surgeons of Columbia UniversityNew YorkUSA

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