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Tobacco Mosaic Virus Structure and Self-Assembly

  • A. C. Bloomer
  • P. J. G. Butler
Part of the The Viruses book series (VIRS)

Abstract

Tobacco mosaic virus (TMV) has become a classical object for studies on the structure and assembly of viruses. Shortly after the first purification of virus by Stanley (1935), structural studies were begun using the methods of biochemistry (Bawden and Pirie, 1937) and X-ray diffraction (Bernal and Fankuchen, 1941). The difficulties encountered in elucidating a structure of this size led to many developments in both techniques and instrumentation which enabled the structure to be determined at steadily increasing resolution. Since the virus has a helical structure (Watson, 1954) and forms highly ordered gels rather than single crystals, the three-dimensional structure must be determined by deconvolution from two-dimensional diffraction patterns of the virus gels which are azimuthally disordered. Notwithstanding the difficulties imposed by this, the virus structure has been solved to a resolution approaching 0.4 nm (Stubbs et al., 1977). In the absence of RNA, the protein will form true crystals of one of its aggregates (the disk; see Section II.B.1) whose structure has been solved to a resolution beyond 3 nm which allowed an atomic model to be built (Bloomer et al., 1978).

Keywords

Coat Protein Tobacco Mosaic Virus Polymorphic Form Cucumber Green Mottle Mosaic Virus Cowpea Chlorotic Mottle Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • A. C. Bloomer
    • 1
  • P. J. G. Butler
    • 1
  1. 1.MRC Laboratory of Molecular BiologyCambridgeEngland

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