Blepharisma (Figs. 1A and 1B) is a genus of ciliate protozoans in which most of the 47 listed species contain a pink pigment named blepharismin that renders them sensitive to glass-transmitted visible and nearultraviolet (UV) radiation. Initially, the pigment was called zoopurpurin by Arcichovskij (1905), who first determined its absorption spectrum. The name was changed to blepharismin to conform to the terminology of hypericin-like pigments, using the genus name as the basis of nomenclature: hypericin from Hypericum, stentorin from Stentor, fabrein from Fabrea, and blepharismin from Blepharisma (Giese, 1973).


Action Spectrum Bright Light Pigment Granule Photodynamic Action Contractile Vacuole 


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  1. Archichovskij, V., 1905, Über das Zoopurpurin, ein neues Pigment der Protozoa (Blepharisma lateritium), (Ehrb), Arch. Protistenkd. 6:227–229.Google Scholar
  2. Blum, H. F., 1941, Photodynamic Action and Diseases Caused by Light, Chemical Monograph Co., Cleveland, Ohio.Google Scholar
  3. Brockmann, H. H., 1952, Photodynamically active natural pigments, in: Progress in Organic Chemistry (J. W. Cook, ed.), Vol. 1, pp. 64–82, Plenum Press, New York.Google Scholar
  4. Brockmann, H. H., 1957, Centenary lecture — Photodynamically active plant pigments, Proc. Chem. Soc. 1957:304–312.Google Scholar
  5. Brockmann, H., Kluge, F., and Maxfeldt, H., 1957, Total Synthese des Hypericins, Chem. Ber. 90:2302–2318.CrossRefGoogle Scholar
  6. Chunosoff, I., Isquith, I. R., and Hirshfield, H. I., 1965, An albino strain of Blepharisma, J. Protozool. 12:459–464.Google Scholar
  7. Epel, B. L., 1973, Inhibition of growth and respiration by visible light and near visible light, in: Photophysiology (A. C. Giese, ed.), Vol. 8, pp. 209–230, Academic Press, New York.Google Scholar
  8. Giese, A. C., 1938a, Reversible bleaching of Blepharisma, Trans. Am. Micros. Soc. 57:77–81.CrossRefGoogle Scholar
  9. Giese, A. C., 1938b, Cannibalism and gigantism in Blepharisma, Trans. Am. Micros. Soc. 57:245–255.CrossRefGoogle Scholar
  10. Giese, A. C., 1938c, Differential susceptibility of a number of protozoans to ultraviolet radiation, J. Cell. Comp. Physiol. 12:129–138.CrossRefGoogle Scholar
  11. Giese, A. C., 1945, The ultraviolet action spectrum for retardation of division of Paramecium, J. Cell. Comp. Physiol. 26:47–55.CrossRefGoogle Scholar
  12. Giese, A. C., 1946, An intracellular photodynamic sensitizer in Blepharisma, J. Cell. Comp. Physiol. 28:119–127.CrossRefGoogle Scholar
  13. Giese, A. C., 1949, A cytotoxin from Blepharisma, Biol. Bull. 97:145–149.CrossRefGoogle Scholar
  14. Giese, A. C., 1953, Some properties of a photodynamic pigment from Blepharisma, J. Gen. Physiol. 37:259–269.CrossRefGoogle Scholar
  15. Giese, A. C., 1957, Photodynamic effect of Blepharisma pigment on nerve, J. Cell. Comp. Physiol. 49:295–302.CrossRefGoogle Scholar
  16. Giese, A. C., 1965, Blepharisma intermedium: Ultraviolet resistance of pigmented and albino clones, Science 149:540–541.CrossRefGoogle Scholar
  17. Giese, A. C., 1967, Effects of radiation on protozoa, in: Research in Protozoology (T. T. Chen, ed.), Vol. 2, pp. 267–357, Pergamon Press, Oxford.Google Scholar
  18. Giese, A. C., 1970, Macromolecular synthesis during regeneration in Blepharisma determined by specific inhibitors and incorporation of 14C-tracers, Exp. Cell. Res. 51:91–102.CrossRefGoogle Scholar
  19. Giese, A. C., 1971a, Nucleic acid synthesis as measured by incorporation of tracers during regeneration in ultraviolet-treated Blepharisma, Exp. Cell. Res. 64:218–226.CrossRefGoogle Scholar
  20. Giese, A. C., 1971b, Photosensitization by natural pigments, in: Photophysiology (A. C. Giese, ed.), Vol. 6, pp. 77–129, Academic Press, New York.Google Scholar
  21. Giese, A. C., 1973, Blepharisma, The Biology of a Light-Sensitive Protozoan, Stanford University Press, Stanford, Calif.Google Scholar
  22. Giese, A. C., 1976, Living with Our Sun’s Ultraviolet Rays, Plenum Press, New York.CrossRefGoogle Scholar
  23. Giese, A. C., 1979, Seasonal photosensitivity of Blepharisma: The role of temperature, Photochem. Photobiol. 29:121–126.CrossRefGoogle Scholar
  24. Giese, A. C., 1980, Hypericism, in: Photochemical and Photobiological Reviews (K. C. Smith, ed.), Vol. 5, pp. 229–255, Plenum Press, New York.Google Scholar
  25. Giese, A. C., and Crossman, F. B., 1945, The action spectrum of sensitization to heat by ultraviolet light, J. Gen. Physiol. 29:79–87.CrossRefGoogle Scholar
  26. Giese, A. C., and Crossman, E. B., 1946, Sensitivation to heat by visible light in presence of photodynamic dyes, J. Gen. Physiol. 29:123–202.CrossRefGoogle Scholar
  27. Giese, A. C., and Grainger, R. M., 1970, Studies on the red and blue forms of the pigment of Blepharisma, Photochem. Photobiol. 12:489–503.CrossRefGoogle Scholar
  28. Giese, A. C., Brandt, C. L., Iverson, R. M., and Wells, P. H., 1952, Photoreactivation in Colpidium colpoda, Biol. Bull. 103:336–344.CrossRefGoogle Scholar
  29. Giese, A. C., and Lusignan, M., 1960, Stimulation of postirradiation recovery of cells by cutting, Science 132:806–807.CrossRefGoogle Scholar
  30. Giese, A. C., and Lusignan, M., 1961, Retardation of regeneration and division of Blepharisma by ultraviolet radiation and its photoreversal, J. Gen. Physiol. 44:543–554.CrossRefGoogle Scholar
  31. Giese, A. C., Iverson, R. M., Shepard, D. C., Jacobson, C., and Brandt, C. L., 1953, Quantum relations in the photoreactivation of Colpidium, J. Gen. Physiol. 37:249–258.CrossRefGoogle Scholar
  32. Giese, A. C., Shepard, D. C., Bennett, J., Farmanfarmaian, A., and Brandt, C. L., 1956, Evidence for thermal reactions following exposure of Didinium to ultraviolet radiation, J. Gen. Physiol. 40:311–325.CrossRefGoogle Scholar
  33. Giese, A. C., and Zeuthen, E., 1949, Photooxidations in pigmented Blepharisma, J. Gen. Physiol. 32:525–535.CrossRefGoogle Scholar
  34. Giese, A. C., McCaw, B. K., and Cornell, R., 1963, Retardation of division of three ciliates by intermittent and continuous ultraviolet radiation at different temperatures, J. Gen. Physiol. 46:1095–1108.CrossRefGoogle Scholar
  35. Giese, A. C., McCaw, B. K., Parker, J. W., and Recher, J. T., 1965, Comparative ultraviolet sensitivity of regeneration in the genus Blepharisma, J. Protozool. 12:171–177.Google Scholar
  36. Hirshfield, H., and Giese, A. C., 1953, Ultraviolet radiation effects of growth processes of Blepharisma undulans, Exp. Cell. Res. 4:283–294.CrossRefGoogle Scholar
  37. Hukai, T., 1968, The effect of ultraviolet radiation on Blepharisma undulans japonicus, Bull. Fukuoka Univ. Educ. Part III Nat. Sci. 18:87–92.Google Scholar
  38. Inaba, F., Nakamura, R., and Yamaguchi, S., 1958, An electron microscopic study on the pigment granules of Blepharisma, Cytologia 23:72–79.CrossRefGoogle Scholar
  39. Jäckie, H., and Kalthoff, K., 1978, Photoreactivation of RNA in UV-irradiated insect eggs (Smittia sp., Chironomidae, Diptera). I. Photosensitized production and lightdependent disappearance of pyrimidine dimers. Photochem. Photobiol. 27:309–315.CrossRefGoogle Scholar
  40. Kennedy, J. R., Jr., 1966, The effect of strychnine and light on pigmentation in Blepharisma undulans Stein, J. Cell. Biol. 28:145–153.CrossRefGoogle Scholar
  41. Kurita, M., 1968, Electron microscopy of the pigment granules in Blepharisma, Biol. J. Nara Women’s Univ. 18:50–52.Google Scholar
  42. Lankester, E. R., 1873, Blue stentorin, the coloring matter in Stentor coeruleus, Q. J. Microscop. Sci. 13:139–142.Google Scholar
  43. Lennartz, D. C., and Bovee, E. C., 1980, The induction of macrostome formation in Blepharisma americanum (Suzuki) by alpha tocopheryl succinate, Trans. Am. Micros. Soc. 99:310–316.CrossRefGoogle Scholar
  44. Longerbeam, D., 1980, The action spectrum for photodynamic killing in Blepharisma, Master’s thesis, San Francisco State University (experimental work done at Sanford).Google Scholar
  45. Miyake, A., and Beyer, J., 1974, Blepharmone: A conjugation-inducing glycoprotein in the ciliate Blepharisma, Science 185:621–623.CrossRefGoogle Scholar
  46. Miyake, A., and Bleyman, L. K., 1976, Gamones and mating types in the genus Blepharisma and their possible taxonomic application, Genet. Res., Cambr. 27:267–275.CrossRefGoogle Scholar
  47. Müller, K. M., 1962, On the nature of stentorin, C. R. Lab. Carlsberg, 32:471–498.Google Scholar
  48. Nadler, J. E., 1929, Notes on loss and regeneration of the pellicle in Blepharisma, Biol. Bull. 56:327–330.CrossRefGoogle Scholar
  49. Parker, J. W., and Giese, A. C., 1966, Nuclear activity during regeneration in Blepharisma intermedium Bhandary, J. Protozool. 13:617–622.Google Scholar
  50. Parker, J. W., and Giese, A. C., 1971, Cytochemical analysis of enzyme activity during regeneration in Blepharisma intermedium Bhandary, J. Protozool. 18:106–115.Google Scholar
  51. Prowazek, S., 1904, Beitrag zur Kenntnis der Regeneration und Biologie der Protozoen, Arch. Protistkd. 3:44–59.Google Scholar
  52. Raab, O., 1900, Ueber die Wirkung fluorescierender Stoffe auf Infusorien, Z. Biol. 39:534–546.Google Scholar
  53. Rao, A. V. S., 1963, Extrusion of a protein-pigment complex from Blepharisma undulans, J. Protozool. 10:204–207.Google Scholar
  54. Rudzinska, M. A., and Granick, S., 1953, Protoporphyrin production of Tetrahymena geleii, Proc. Soc. Exp. Biol. Med. 83:525–526.Google Scholar
  55. Schorr, L., 1961, An action spectrum of ultraviolet effects of dividing Blepharisma undulans, Diss. Abstr. 22:899–900.Google Scholar
  56. Seshachar, B. R., Rao, A. V. S. P., and Padmavathi, P. B., 1957, Properties of the pigment of an Indian species of Blepharisma, J. Sci. Ind. Res. 16C:201–204.Google Scholar
  57. Sevenants, M. R., 1965, Pigments of Blepharisma compared with hypericin, J. Protozool. 12:240–245.Google Scholar
  58. Smith, E. M., and Giese, A. C., 1975, Photoreactivation in Blepharisma, Exp. Cell. Res. 90:448–454.CrossRefGoogle Scholar
  59. Smith, K. C., 1977a, New topics in photobiology, in: The Science of Photobiology (K. C. Smith, ed.), pp. 397–417, Plenum Press, New York.CrossRefGoogle Scholar
  60. Smith, K. C., 1977b, Ultraviolet radiation effects on molecules and cells, in: The Science of Photobiology (K. C. Smith, ed.), pp. 113–142, Plenum Press, New York.CrossRefGoogle Scholar
  61. Smith, S., and Giese, A. C., 1967, Axenic media for Blepharisma intermedium, J. Protozool. 14:649–654.Google Scholar
  62. Tartar, V., 1961, The Biology of Stentor, Pergamon Press, New York.Google Scholar
  63. Tartar, V., 1972, Caffeine bleaching of Stentor, J. Exp. Zool. 181:245–252.CrossRefGoogle Scholar
  64. Utsumi, K., 1953, Some properties of the pigment in Blepharisma, J. Sci. Hiroshima Univ. Ser. B, Div. I, Zool. 14:35–38.Google Scholar
  65. Utsumi, K., and Yoshizawa, K., 1957, Intracellular structure of Blepharisma as revealed by electron microscope, Zool. Mag. 66:234–239.Google Scholar
  66. Walker, E. B., Lee, T. Y., and Song, P. S., 1979, Spectroscopic characterization of the Stentor photoreceptor, Biochim. Biophys. Acta 587:129–144.CrossRefGoogle Scholar
  67. Weisz, P. B., 1950, On the mitochondrial nature of the pigmented granules in Stentor and Blepharisma, J. Morphol. 86:177–184.CrossRefGoogle Scholar
  68. Wood, D. C., 1976, Electrophysiological responses correlated with the Photophobie response of Stentor coeruleus, Photochem. Photobiol. 24:261–266.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1981

Authors and Affiliations

  • Arthur C. Giese
    • 1
  1. 1.Department of Biological SciencesStanford UniversityStanfordUSA

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