Advertisement

Sister Chromatid Exchange in Human Lymphocytes as an Indicator of DNA Damage and Repair in Vivo

  • Bo Lambert

Abstract

The development of staining techniques for the demonstration of sister chromatid exchanges (SCE) (1) has introduced a new, promising tool in the evaluation of genotoxic effects of mutagenic and carcinogenic agents. SCEs arise when the two chromatids within a single chromosome exchange segments (Fig. 1). This usually occurs at homologous loci, so the SCE itself may not alter the genetic information of the cell or its daughter cells. In fact, SCEs may represent a natural event of mitotic recombination, since present evidence suggests that SCE occurs at a very low frequency in normal somatic cells (2).

Keywords

Ethylene Oxide Ethylene Oxide Human Lymphocyte Chromosome Aberration Heavy Smoker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Latt, S.A., Microfluorimetric detection of DNA synthesis in human chromosomes, Proc. Nat. Acad. Sci. 70:3395 (1973).PubMedCrossRefGoogle Scholar
  2. 2.
    Kato, H., Spontaneous sister chromatid exchanges detected by a BUdR-labelling method, Nature 251:70 (1974).PubMedCrossRefGoogle Scholar
  3. 3.
    Wolff, S., Bodycote, J. and Painter, R.B., Sister chromatid exchanges induced in Chinese hamster cells by UV-irradiation of different stages of the cell cycle: the necessity for cells to pass through S, Mutation Res. 25:73 (1974).PubMedCrossRefGoogle Scholar
  4. 4.
    Wolff, S., Chromosomal effects of mutagenic carcinogens and the nature of the lesions leading to sister chromatid exchange, in “Mutagen induced chromosome damage in man,” H.J. Evans and D.C. Lloyd (eds.), Edinburgh University Press, Edinburgh (1978).Google Scholar
  5. 5.
    Evans, H.J., Molecular mechanisms in the induction of chromosome aberrations, in “Progress in genetic toxicology,” B.A. Bridges, D. Scott and F.H. Sobels (eds.), Elsevier North Holland, Amsterdam (1977).Google Scholar
  6. 6.
    Perry, P. and Evans, H.J., Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange, Nature 258: 121 (1975).PubMedCrossRefGoogle Scholar
  7. 7.
    Solomon, E. and Bobrow, M., Sister chromatid exchanges — a sensitive assay of agents damaging human chromosomes, Mutation Res., 30:273 (1975).PubMedGoogle Scholar
  8. 8.
    Popescu, N.C., Turnbull, D. and di Paolo, J.A., Sister chromatid exchange and chromosome aberration analysis with the use of several carcinogens and non-carcinogens, J. Natl. Cancer Inst. 59:289 (1977).PubMedGoogle Scholar
  9. 9.
    Abe, S. and Sasaki, M., Chromosome aberrations and sister chromatid exchanges in Chinese hamster cells exposed to various chemicals, J. Natl. Cancer Inst., 58:1635 (1977).PubMedGoogle Scholar
  10. 10.
    Takehisa, S. and Wolff, S., Induction of sister chromatid exchanges in Chinese hamster cells by carcinogenic mutagens requiring metabolic activation, Mutation Res., 45:263 (1977).PubMedCrossRefGoogle Scholar
  11. 11.
    Vogel, W. and Bauknecht, Th., Differential chromatid staining by in vivo treatment as a mutagenic test system, Nature, 260:448 (1976).PubMedCrossRefGoogle Scholar
  12. 12.
    Marquardt, H. and Bayer, U., The induction of sister chromatid exchanges in the bone marrow of the Chinese hamster, I: The sensitivity of the system (methyl methane sulphonate), Mutation Res. 56:169 (1977).CrossRefGoogle Scholar
  13. 13.
    Allen, J.W., Schuler, C.F., Mendes, R.W. and Latt, S.A., A simplified technique for in vivo analysis of sister chromatid exchanges using 5-bromodeoxyuridine tablets, Cytogenet. Cell Genet. 18:231 (1977).PubMedCrossRefGoogle Scholar
  14. 14.
    Carrano, A.V., Thompson, L.H., Lindl, P.A. and Minkler, J.L., Sister chromatid exchange as an indicator of mutagenesis, Nature 271:551 (1978).PubMedCrossRefGoogle Scholar
  15. 15.
    Perry, P. and Wolff, S., New Giemsa method for the differential staining of sister chromatids, Nature 251:156 (1974).PubMedCrossRefGoogle Scholar
  16. 16.
    Lambert, B., Hansson, K., Lindsten, J., Sten, M. and Werelius, B., Bromodeoxyuridine-induced sister chromatid exchanges in human lymphocytes, Hereditas 83:163 (1976).PubMedCrossRefGoogle Scholar
  17. 17.
    Lambert, B., Lindblad, A., Nordenskjöld, M. and Werelius, B., Increased frequency of sister chromatid exchanges in cigarette smokers, Hereditas 88:147 (1978).PubMedCrossRefGoogle Scholar
  18. 18.
    Crossen, P.E., Drets, M.E., Arrighi, F.E. and Johnston, D.A., Analysis of the frequency and distribution of sister chromatid exchanges in human lymphocytes, Hum. Genetics 35:345 (1977).CrossRefGoogle Scholar
  19. 19.
    Morgan, W.F. and Crossen, P.E., The incidence of sister chromatid exchanges in cultured human lymphocytes, Mutation Res. 42:305 (1977).PubMedCrossRefGoogle Scholar
  20. 20.
    Parrish, J.A., Fitzpatrick, T.B., Tannenbaum, L. and Pathak, M.A., Photochemotherapy of psoriasis with oral methoxsalen and longwave ultraviolet light, N. Engl. J. Med. 291:1207 (1974).PubMedCrossRefGoogle Scholar
  21. 21.
    Swanbeck, G., Thyresson-Hök, M., Bredberg, A. and Lambert, B., Treatment of psoriasis with oral psoralens and longwave ultraviolet light: therapeutic results and cytogenetic hazards, Acta Dermatovener (Stockholm) 56:367 (1975).Google Scholar
  22. 22.
    Scott, B.R., Pathak, M.A. and Mohn, G.R., Molecular and genetic basis of furocoumarin reactions, Mutation Res. 39:29 (1976).PubMedGoogle Scholar
  23. 23.
    Lambert, B., Morad, M., Bredberg, A., Swanbeck, G. and Thyresson-Hök, M., Sister chromatid exchanges in lymphocytes from psoriasis patients treated with 8-methoxypsoralen and longwave ultraviolet light, Acta Dermatovener, (Stockholm), 58:13 (1978).Google Scholar
  24. 24.
    Lambert, B., Ringborg, U., Harper, E. and Lindblad, A., Increased frequency of sister chromatid exchanges in patients receiving chemotherapy against malignant disorders, Cancer Treatment Reports, 62: 1413 (1978).PubMedGoogle Scholar
  25. 25.
    Lambert, B., Ringborg, U. and Lindblad, A., Prolonged increase of sister chromatid exchanges in lymphocytes of melanoma patients after CCNU treatment, Mutation Res., 59:295 (1979).PubMedCrossRefGoogle Scholar
  26. 26.
    Funes-Cravioto, F., Zapata-Gayon, C., Kolmodin-Hedman, B., Lambert, B., Lindsten, J., Norberg, E., Nordenskjöld, M., Olin, R. and Swensson, A., Chromosomal aberrations and sister chromatid exchange in workers in chemical laboratories and a rotoprinting factory and in children of women laboratory workers, Lancet II:322 (1977).CrossRefGoogle Scholar
  27. 27.
    Funes-Cravioto, F., Zapata-Gayon, C., Kolmodin-Hedman, B., Lambert, B., Lindsten, J., Norberg, E., Nordenskjöld, M., Olin, R. and Swensson, A., Chromosome aberrations and sister chromatid exchange in laboratory and factory workers and their children, in “Mutagen induced chromosome damage in man,” H.J. Evans and D.C. Lloyd (eds.) p. 275, Edinburgh University Press, Edinburgh (1978).Google Scholar
  28. 28.
    Ehrenberg, L., Hiesche, K.D., Osterman-Golkar, S. and Wennberg, J., Evaluation of genetic risks of alkylating agents: tissue doses in the mouse from air contaminated with ethylene oxide, Mutation Res., 24:83 (1974).PubMedCrossRefGoogle Scholar
  29. 29.
    Calleman, C.J., Ehrenberg, L., Jansson, B., Osterman-Golkar, S., Segerbäck, D., Svensson, K. and Wachtmeister, C.A., Monitoring and risk assessment by means of alkyl groups in haemoglobin in persons occupationally exposed to ethylene oxide, J. Toxicol. Environm. Health, (1978) (in press).Google Scholar
  30. 30.
    Lambert, B., Lindblad, A., Hansson, K., Calleman, C.J., Ehrenberg, L. and Osterman-Golkar, S., Increased frequency of sister chromatid exchanges in human subjects exposed to ethylene oxide (1978) (to be published).Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Bo Lambert
    • 1
  1. 1.Department of Clinical GeneticsKarolinska HospitalStockholmSweden

Personalised recommendations