Screening for Deficits in DNA Repair Using Human Lymphocytes
With the growing awareness concerning environmental carcinogenesis, it has become important to develop methods to quantitate the response of individuals to carcinogens. With these tests in hand, there will be at least three important distinct areas requiring extensive investigation: 1) the determination of which chemicals in the environment initiate and promote malignant changes; 2) the delineation of groups of individuals having genetic differences in their response to particular carcinogens; and 3) the development of methods to quantitate damage to cellular DNA which has incurred from prior exposure to carcinogens. Tests to screen for chemical carcinogens based on mutagenicity have been established in tissue culture, in bacteria, and in cell-free systems. The definitive carcinogenicity of environmental agents must ultimately rest on the results from animal test systems and from epidemiological data in human populations. For measuring genetic variations in the response of individuals to carcinogens and possibly for analyzing accumulated damage to DNA, human peripheral lymphocytes are a particularly advantageous biological system.
KeywordsThymidine Incorporation Xeroderma Pigmentosum Ataxia Telangiectasia Unirradiated Control Human Peripheral Lymphocyte
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