Functions of DNA Polymerases α, β and γ in DNA Replication and Repair

  • Miria Stefanini
  • Anna I. Scovassi
  • Umberto Bertazzoni


The cells of vertebrate organisms contain three distinct DNA polymerases which have been designed α, β and γ -polymerases (1). The information concerning the physical, chemical and catalytical properties of these enzymes has progressed considerably during the past few years so that the distinction of their activities is easily obtained by utilizing the differences in molecular weight, sensitivity to inhibitors, chromatographic elution, ability to copy various templates (reviews: 2–8). The general properties of the three enzymes are summarized in Table I.


Irradiate Cell Xeroderma Pigmentosum Molecular Weight Enzyme Human Heteroploid Cell Irradiate Cell Sample 
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  1. (1).
    Weissbach, A., Baltimore, D., Bollum, F.J., Gallo, R. C. and Korn, D. (1975) Science 190:401–402.PubMedCrossRefGoogle Scholar
  2. (2).
    Bollum, F.J. (1975) Prog. Nucl. Acid Res. and Mol. Biol. 15:109–144.CrossRefGoogle Scholar
  3. (3).
    Holmes, A.M. and Johnston, I. R. (1975) FEBS Letters 60:233–243.PubMedCrossRefGoogle Scholar
  4. (4).
    Weissbach, A. (1977) Ann. Rev. Biochem. 46:25–47.PubMedCrossRefGoogle Scholar
  5. (5).
    Wintersberger, E. (1977) TIBS 58–61.Google Scholar
  6. (6).
    Falaschi, A. and Spadari, S. (1978) from: DNA SYNTHESIS: Present and Future, Edited by I. Molineux and M. Kohiyama (Plenum Publishing Corporation, 1978).Google Scholar
  7. (7).
    Dube, D.K., Travaglini, E.C. and Loeb, L. A. (1978) Methods in Cell Biology XIX. Ed. by G. Stein, J. Stein, L. J. Kleinsmith.Google Scholar
  8. (8).
    Sarngadharan, M. G., Robert-Guroff, M. and Gallo, R. C. (1978) Biochim. Biophys. Acta 516:419–487.PubMedGoogle Scholar
  9. (9).
    Bollum, F.J. (1979) from: Antiviral Mechanisms for the control of neoplasia, ed. by E. S. H. Prakash, Plenum Publishing Co. (1979).Google Scholar
  10. (10).
    Ohashi, M., Taguchi, T. and Ikegami, S. (1978) Biochem. Biophys. Res. Comm. 82:1084–1090.PubMedCrossRefGoogle Scholar
  11. (11).
    Edenberg, H. J., Anderson, S. and De Pamphilis, M.L. (1978) J. Biol. Chem. 253:3273–3280.PubMedGoogle Scholar
  12. (12).
    Chang, L.M.S. (1973) J. Biol. Chem. 248:3789–3795.PubMedGoogle Scholar
  13. (13).
    Wang, T.S-F., Sedwick, W.D. and Korn, D. (1975) J. Biol. Chem. 250:7040–7044.PubMedGoogle Scholar
  14. (14).
    Chang, L.M.S. (1976) Science 191:1183–1185.PubMedCrossRefGoogle Scholar
  15. (15).
    Knopf, K.W., Yamada, M. and Weissbach, A. (1976) Biochemistry 15:4540–4548.PubMedCrossRefGoogle Scholar
  16. (16).
    Bolden, A., Pedrali-Noy, G. and Weissbach, A. (1977) J. Biol. Chem. 252:3351–3356.PubMedGoogle Scholar
  17. (17).
    Bertazzoni, U., Scovassi, A.I. and Brun, G. (1977) Eur. J. Biochem. 81:237–248.PubMedCrossRefGoogle Scholar
  18. (18).
    Hübscher, U., Kuenzle, C.C. and Spadari, S. (1977) Eur. J. Biochem. 81:249–258.PubMedCrossRefGoogle Scholar
  19. (19).
    Tarrago-Litvak, L., Viratelle, O., Darriet, D., Dalibart, R., Graves, P.V. and Litvak, S. (1978) Nucleic Acid Res. 5:2197–2210.PubMedCrossRefGoogle Scholar
  20. (20).
    Bollum, F.J. and Potter, V.R. (1957) JACS 79:3603.CrossRefGoogle Scholar
  21. (21).
    Loeb, L.A., Agarwal, S.S. and Woodside, A.M. (1968) Proc. Natl. Acad. Sci. USA 61:827–834.PubMedCrossRefGoogle Scholar
  22. (22).
    Rabinowitz, Y., McCluskey, I. S., Wong, P. and Wilhite, B.A. (1969) Exp. Cell Res. 57:257–262.PubMedCrossRefGoogle Scholar
  23. (23).
    Pedrini, A.M., Nuzzo, F., Ciarrocchi, G., Dalprà, L. and Falaschi, A. (1972) Biochem. Biophys. Res. Commun. 47:1221–1227.PubMedCrossRefGoogle Scholar
  24. (24).
    Tyrsted, G., Munch-Petersen, B. and Cloos, L. (1973) Exp. Cell Res. 77:415–427.PubMedCrossRefGoogle Scholar
  25. (25).
    Chang, L.M.S. and Bollum, F.J. (1972) J. Biol. Chem. 247:7948–7950.PubMedGoogle Scholar
  26. (26).
    Chang, L.M.S., Brown, Mc.K. and Bollum, F.J. (1973) J. Mol. Biol. 74:1–8.PubMedCrossRefGoogle Scholar
  27. (27).
    Coleman, M.S., Hutton, I. J. and Bollum, F.J. (1974) Nature 248:407–409.PubMedCrossRefGoogle Scholar
  28. (28).
    Roodman, G. D., Hutton, J. J. and Bollum, F.J. (1976) Biochim. Biophys. Acta 425:478–491.PubMedGoogle Scholar
  29. (29).
    Spadari, S., Villani, G. and Hardt, N. (1978) Exp. Cell Res. 113:57–62.PubMedCrossRefGoogle Scholar
  30. (30).
    Spadari, S. and Weissbach, A. (1974) J. Mol. Biol. 86:11–20.PubMedCrossRefGoogle Scholar
  31. (31).
    Chiu, R.W. and Baril, E.F. (1975) J. Biol. Chem. 250:7951–7957.PubMedGoogle Scholar
  32. (32).
    Hübscher, U., Kuenzle, C.C. and Spadari, S. (1977) Nucleic Acids Res. 4:2917–2929.PubMedCrossRefGoogle Scholar
  33. (33).
    Otto, B. and Fanning, E. (1978) Nucleic Acids Res. 5:1715–1728.PubMedCrossRefGoogle Scholar
  34. (34).
    Mechali, M., Girard, M. and de Recondo, A.M. (1977) J. Virology 23:117–125.PubMedGoogle Scholar
  35. (35).
    Waqar, M.A., Evans, M.J. and Huberman, J. A. (1978) Nucleic Acids Res. 5:1933–1946.PubMedCrossRefGoogle Scholar
  36. (36).
    Ikegami, S., Taguchi, T., Ohashi, M., Oguro, M., Nagano, H. and Mano, Y. (1978) Nature 275:458–460.PubMedCrossRefGoogle Scholar
  37. (37).
    Spadari, S. and Weissbach, A. (1975) Proc. Natl. Acad. Sci. USA 72:503–507.PubMedCrossRefGoogle Scholar
  38. (38).
    Edenberg, H. J. and Huberman, J. A. (1975) Ann. Rev. Genetics 9:245–284.CrossRefGoogle Scholar
  39. (39).
    Eliasson, R. and Reichard, P. (1978) Nature 272:184–185.PubMedCrossRefGoogle Scholar
  40. (40).
    Brun, G. and Weissbach, A. (1978) Proc. Natl. Acad. Sci. USA 75:5931–5935.PubMedCrossRefGoogle Scholar
  41. (41).
    Herrick, G., Delius, A. and Alberts, B. (1976) J. Biol. Chem. 251:2142–2146.PubMedGoogle Scholar
  42. (42).
    Otto, B., Baynes, M. and Knippers, R. (1977) Eur. J. Biochem. 73:17–24.PubMedCrossRefGoogle Scholar
  43. (43).
    Cobianchi, F., Riva, S., Mastromei, G., Spadari, S., Pedrali, G. and Falaschi, A. (1978) Cold Spring Harbor Symp. Quant. Biol., vol. 43 “DNA: Replication and Recombination”, in press.Google Scholar
  44. (44).
    Hübscher, U., Kuenzle, C.C. and Spadari, S. (1979) Proc. Natl. Acad. Sci. USA, in press.Google Scholar
  45. (45).
    Bertazzoni, U., Stefanini, M., Pedrali-Noy, G., Giulotto, E., Nuzzo, F., Falaschi, A. and Spadari, S. (1976) Proc. Natl. Acad. Sci. USA 73:785–789.PubMedCrossRefGoogle Scholar
  46. (46).
    Pedrali-Noy, G., Dalprà, L., Pedrini, A.M., Ciarrocchi, G., Giulotto, E., Nuzzo, F. and Falaschi, A. (1974) Nucleic Acids Res. 1:1183–1200.CrossRefGoogle Scholar
  47. (47).
    Dalprà, L., Stefanini, M., Giulotto, E., Falaschi, and Nuzzo, F. (1978) Haematologica 64:31–39.Google Scholar
  48. (48).
    Hecht, N.B., Farrell, D. and Davidson, D. (1976) Dev. Biol. 48:56–66.PubMedCrossRefGoogle Scholar
  49. (49).
    Ringborg, U. and Lambert, B. (1977) Cancer Letters 3:77–81.PubMedCrossRefGoogle Scholar
  50. (50).
    Coleman, M.S., Hutton, J.J. and Bollum, F.J. (1974) Blood 44:19–32.PubMedGoogle Scholar
  51. (51).
    Smith, G. J., Charlton, R. K., Grisham, J.W. and Kaufman, D. (1978) Biochem. Biophys. Res. Comm. 4:1538–1544.CrossRefGoogle Scholar
  52. (52).
    Fisher, P.A., Wang, T.S.F. and Korn, D. (1978) Cold Spring Harbor Symp. Quant. Biol. vol. 43 “DNA Replication and Recombination” in press.Google Scholar
  53. (53).
    Coetzee, M.L., Chou, R. and Ove, P. (1978) Cancer Res. 38:3621–3627.PubMedGoogle Scholar
  54. (54).
    Setlow, R. B. (1978) Nature 271:713–717.PubMedCrossRefGoogle Scholar
  55. (55).
    Arlett, C. and Lehmann, A. (1978) Ann.Rev.Genet. 12:95–115.PubMedCrossRefGoogle Scholar
  56. (56).
    Bertazzoni, U., Stefanini, M., Pedrali-Noy, G., Nuzzo, F., and Falaschi, A. (1977) Nucleic Acids Res. 4:141–148.PubMedCrossRefGoogle Scholar
  57. (57).
    Bertazzoni, U., Scovassi, A.I, Stefanini, M., Giulotto, E., Spadari, S. and Pedrini, M.A. (1978) Nucleic Acids Res. 5:2189–2196.PubMedCrossRefGoogle Scholar
  58. (58).
    Parker, V.P. and Lieberman, M.W. (1977) Nucleic Acids Res. 4:2029–2037.PubMedCrossRefGoogle Scholar
  59. (59).
    Wicker, R., Scovassi, A.I. and Nocentini, S. (1978) submitted for publication.Google Scholar
  60. (60).
    Mezzina, M. and Nocentini, S. (1978) Nucleic Acids Res. 5:4317–4328.PubMedCrossRefGoogle Scholar
  61. (61).
    Clayton, D. A., Doda, J.M. and Friedberg, E. C. (1974) Proc.Natl. Acad. Sci. USA 71:2777–2781.PubMedCrossRefGoogle Scholar
  62. (62).
    Bray, G. and Brent, T.P. (1972) Biochim. Biophys. Acta 269:184–191.PubMedGoogle Scholar
  63. (63).
    Villani, G., Defais, M., Spadari, S., Caillet-Fauquet, P., Boiteux, S. and Radman, M. (1977) from “Research in Photobiology” ed. by A. Castellani, Plenum Publishing Co., New York.Google Scholar
  64. (64).
    Loeb, L. (1979) this volume.Google Scholar
  65. (65).
    Campagnari, F., Bertazzoni, U. and Clerici, L. (1967) J. Biol. Chem. 242:2168–2171.PubMedGoogle Scholar
  66. (66).
    Radman, M., Villani, G., Boiteux, S., Kinsella, A. R., Glickman, B.W. and Spadari, S. (1978) Cold Spring Harbor Symp. Quant. Biol., vol. 43 “DNA: Replication and Recombination”, in press.Google Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Miria Stefanini
    • 1
  • Anna I. Scovassi
    • 1
  • Umberto Bertazzoni
    • 1
  1. 1.Laboratorio di Genetica Biochimica ed EvoluzionisticaConsiglio Nazionale delle RicerchePaviaItaly

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