Thymocyte Maturation Induced by a Cyclic AMP-Elevating Thymic Factor
The differentiation of T lymphocytes into mature immunocompetent cells is probably at least partially under the influence of factors secreted by the thymus, as suggested by experiments in a diffusion chamber (1, 2). Several factors, acting on T-cell differentiation, have been found in thymic extracts, in peripheral blood and in supernatants of thymic epithelial cultures (for review, see ref. 3). Probably, these factors act via specific membrane receptors. Indirect evidence (4) suggests that cyclic AMP (cAMP) may play a’ second messenger’ role in the induction of T-cell differentiation by thymic factors. A thymic humoral factor (THF) (5) and a thymic epithelial culture supernatant (TES) (6) also induce an increase in intracellular cAMP levels in thymocytes. By means of direct measurement of intracellular cAMP in human and mouse thymocytes in vitro, we demonstrated the existence in human serum of a thymus-dependent factor (SF) (7) which has the capacity to increase cAMP levels.
KeywordsTerminal Deoxynucleotidyl Transferase Primary Immunodeficiency Disease Intracellular cAMP Level Mouse Thymocyte Immature Thymocyte
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- 1.Stutman, O. and Good, R.A. Thymus hormones. In: Contemporary Topics in Immunobiology. A.J.S. Davies and R.L. Carter, editors. Plenum Press, New York, Vol. 2, 299 (1973).Google Scholar
- 6.Kruisbeek, A.M., Astaldi, G.C.B., Blankwater, M.J., Zijlstra, J.L., Levert, L.A. and Astaldi, A. The in vitro effect of a thymic epithelial culture supernatant on mixed lymphocyte reactivity and intracellular cAMP levels of thymocytes and on antibody production to SRBC by Nu/Nu spleen cells. Cell. Immunol. 35, 134 (1978).PubMedCrossRefGoogle Scholar
- 10.Wijermans, P. and Astaldi, A. Effect of aging on thymus-dependent serum factor(s). Ned. T. Geront. 9, 216 (1978).Google Scholar
- 11.Wijermans, P., Oosterhuis, H.J.G.H., Astaldi, G.C.B., Schellekens, P.Th.A. and Astaldi, A. Thymus-dependent serum factor in non-thymectomized and thymectomized myasthenia gravis patients. Submitted for publication.Google Scholar
- 12.Wijermans, P., Oosterhuis, H.J.G.H., Astaldi, G.C.B., Schellekens, P.Th.A. and Astaldi, A. Influence of adult thymectomy on the immunocompetence in man. Submitted for publication.Google Scholar
- 17.Astaldi, A., Astaldi, G.C.B., Wijermans, P., Groenewoud, M., Bemmel, T. van, Schellekens, P.Th.A. and Eijsvoogel, V.P. Thymus-dependent human serum factor active on precursors of mature T. cells. In: Proc. 12th Leukocyte Culture Conference. M. Quastel, editor. Academic Press, Inc., New York, in press.Google Scholar
- 26.Facchini, A., Astaldi, G.C.B., Cocco, L., Wijermans, P., Manzoli, F.A. and Astaldi, A. Early events in thymocyte activation. II. Changes in non-histone chromatin proteins induced by a thymus-dependent human serum factor. Submitted for publication.Google Scholar
- 27.Astaldi, G.C.B., Astaldi, A., Wijermans, P., Schellekens, P.Th.A. and Eijsvoogel, V.P. A thymus-dependent serum factor induces maturation of thymocytes as evaluated by Graft-versus-Host reaction. Submitted for publication.Google Scholar
- 33.Bollum, F.J. Terminal deoxynucleotidyl transferase. In: The Enzymes. R.D. Boyer, editor. Academic Press, Inc., New York, Vol. 10, pp. 145–171 (1974).Google Scholar
- 34.Baltimore, D., Silverstone, A.E., Kung, P.C., Harrison, T.A. and McCaffrey, R.P. What cells contain terminal transferase? In: The Generation of Antibody Diversity: a new look. A. Cunningham, editor. Academic Press, Inc., New York, pp. 21–30 (1977).Google Scholar
- 36.Astaldi, G.C.B., Astaldi, A., Wijermans, P., Bemmel, T. van, Schellekens, P.Th.A. and Eijsvoogel, V.P. A thymus-dependent human serum factor induces decrease of terminal deoxynucleotidyl transferase in thymocytes. Submitted for publication.Google Scholar
- 37.Simonsen, M. Graft-versus-host reactions. Their natural history and applicability as tools of research. Progr. Allergy 6, 349 (1962).Google Scholar