Enzymes from Calf Thymus That Might Be Involved in DNA Repair

  • Francesco Campagnari


The DNA molecules carrying the genetic information of cells are susceptible to damage by radiation of certain wavelengths, such as X-, γ- and UV-rays (see for review: Latarjet, 1972; Blok and Loman, 1974; Murphy, 1974), and by organic chemicals that themselves or through their metabolic products react with nucleic acids as strong electrophiles (Miller and Miller, 1966; Miller, 1970). Living cells exposed to these noxious agents undergo functional inactivation, enhancement of the mutation rate and decrease of survival. In vertebrate animals the lesions of DNA may initiate complex processes of cell transformation that will eventually lead to cancer. Radiation and substances that directly or indirectly alter the molecules of DNA are potentially mutagenic and carcinogenic, thus behaving as genetic toxins. Detrimental agents of this type are natural or artificial components of the environment in which we all presently live.


Excision Repair Xeroderma Pigmentosum Pyrimidine Dimer Thymine Dimer Postreplication Repair 
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  1. Bacchetti, S., and Benne, R. (1975) Purification and characterization of an endonuclease from calf thymus acting on irradiated DNA, Biochem. Biophys. Acta 390, 285–297.PubMedGoogle Scholar
  2. Bekkering-Kuylaars, S.A.M., and Campagnari, F. (1974) Characterization and properties of a DNA polymerase partially purified from the nuclei of calf thymus cells, Biochem. Biophys. Acta 349, 277–295.PubMedGoogle Scholar
  3. Bekkering-Kuylaars, S.A.M., and Campagnari, F. (1972) Purification of a DNA polymerase from calf thymus nuclei, Biochem. Biophys. Acta 272, 526–538.PubMedGoogle Scholar
  4. Bernardi, G. (1971) Spleen acid deoxyribonuclease, in “The Enzymes” (P.D. Boyer, ed.), vol. 4, pp. 271–287, Academic Press, New York.Google Scholar
  5. Bertazzoni, U., Mathelet, M., and Campagnari, F. (1972) Purification and properties of a polynucleotide ligase from calf thymus glands, Biochem. Biophys. Acta 287, 404–414.PubMedGoogle Scholar
  6. Blok, J., and Loman, H. (1973) The effects of γ-radiation in DNA, Current Topics Radiat. Res. Quart. 9, 165–245.Google Scholar
  7. Bollum, F.J., Chang, L.M.S., Tsiapalis, C.M., and Dorson, J.W. (1974) Nucleotide polymerizing enzymes from calf thymus glands, Methods Enzymol. 29, 70–81.PubMedCrossRefGoogle Scholar
  8. Bollum, F.J. (1975) Mammalian DNA polymerases, Progr. Nucl. Acid Res. Mol. Biol. 15, 109–114.CrossRefGoogle Scholar
  9. Bollum, F.J., and Setlow, R.B. (1963) Ultraviolet inactivation of DNA primer activity. I. Effects of different wavelengths and doses, Biochem. Biophys. Acta 68, 599–607.PubMedCrossRefGoogle Scholar
  10. Bootsma, D. (1978) Xeroderma pigmentosum, in “DNA Repair Mechanisms” (P.C. Hanawalt, E.C. Friedberg, and C.F. Fox, eds.) pp. 589–601, Academic Press, New York.Google Scholar
  11. Brent, T.P. (1979) Partial purification and characterization of a human 3-methyladenine-DNA glycosylase, Biochemistry 18, 911–916.PubMedCrossRefGoogle Scholar
  12. Campagnari, F., Bertazzoni, U., and Clerici, L. (1967) The priming activity of X-irradiated deoxyribonucleic acid for the deoxyribonucleic acid polymerase from calf thymus, J. Biol. Chem. 242, 2168–2171.PubMedGoogle Scholar
  13. Campagnari, F., and Bertazzoni, U. (1968) Effect of low doses of X-rays on isolated deoxyribonucleic acid (DNA). II. Results obtained by enzymatic methods, Medic. Nucl. Radiobiol. Latina 3, Suppl. 3, 304–311.Google Scholar
  14. Chang, L.M.S., and Bollum, F.J. (1973) A comparison of associated enzyme activities in various deoxyribonucleic acid polymerases, J. Biol. Chem. 248, 3398–3404.PubMedGoogle Scholar
  15. Chang, L.M.S., and Bollum, F.J. (1972) A chemical model for transcriptional initiation of DNA replication, Biochem. Biophys. Res. Commun. 46, 1354–1360.PubMedCrossRefGoogle Scholar
  16. Chang, L.M.S. (1973) Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. I. Preparation of homogeneous enzyme, J. Biol. Chem. 248, 3789–3795.PubMedGoogle Scholar
  17. Chang, L.M.S. (1973) Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. II. Initiation and fidelity of homopolymer replication, J. Biol. Chem. 248, 6983–6992.PubMedGoogle Scholar
  18. Chang, L.M.S. (1975) The distributive nature of enzymatic DNA synthesis, J. Mol. Biol. 93, 219–235.PubMedCrossRefGoogle Scholar
  19. Clark, A.J., and Ganesan, A. (1975) List of genes affecting DNA metabolism in Escherichia coli, in “Molecular Mechanisms of Repair of DNA” (P.C. Hanawalt, and R.B. Setlow, eds.) pp. 431–437, Plenum Press, New York.Google Scholar
  20. Cleaver, J.E. (1968) Defective repair replication of DNA in xeroderma pigmentosum, Nature 218, 652–656.PubMedCrossRefGoogle Scholar
  21. Cleaver, J.E. (1978) DNA repair and its coupling to DNA replication in eukaryotic cells, Biochem. Biophys. Acta 516, 489–516.PubMedGoogle Scholar
  22. Clerici, L., Sponza, G., Talpaert-Borlé, M., and Campagnari, F. (1980) Proofreading exonuclease activity in crude and partially purified preparations of DNA polymerase a from calf thymus, in “DNA Repair and Late Effects” (E. Riklis, H. Altman, and H. Slor, eds.) pp. 23–30, Nuclear Research Center Negev Publisher, Israel.Google Scholar
  23. Cone, R., Duncan, J., Hamilton, L., and Friedberg, E.C. (1977) Partial purification and characterization of a uracil DNA N-glycosydase from Bacillus subtilis, Biochemistry 16, 3194–3201.PubMedCrossRefGoogle Scholar
  24. Corry, P.M., and Cole, A. (1973) Double strand rejoining in mammalian DNA, Nature New Biol. 245, 100–101.PubMedGoogle Scholar
  25. Deutsch, W.A., and Linn, S. (1979) DNA binding activity from cultured human fibroblasts that is specific for partially depurinated DNA and that inserts purines into apurinic sites, Proc. Nat. Acad. Sci. USA 76, 141–144.PubMedCrossRefGoogle Scholar
  26. Fox, M., and Fox, B.W. (1973) Repair replication in X-irradiated lymphoma cells in vitro, Int. J. Radiat. Biol. 23, 333–358.CrossRefGoogle Scholar
  27. Grossman, L., Braun, A., Feldberg, R., and Mahler, I. (1975) Enzymatic repair of DNA, Ann. Rev. Biochem. 44, 19–43.PubMedCrossRefGoogle Scholar
  28. Hanawalt, P.C. (1977) DNA repair processes: an overview, in “DNA Repair Processes” (W.W. Nichols, and D.G. Murphy, eds.) pp. 1–19, Symposia Specialists, Inc., Miami.Google Scholar
  29. Hart, R.W. (1976) Role of DNA repair in aging, in “Aging, Carcinogenesis and Radiation Biology” (K.C. Smith, ed.) pp. 537–556, Plenum Press, New York.Google Scholar
  30. Higgins, N.P., Kato, K., and Strauss, B. (1976) A model for replication repair in mammalian cells, J. Mol. Biol. 101, 417–425.PubMedCrossRefGoogle Scholar
  31. Hill, R.T. (1958) A radiation-sensitive mutant of Escherichia coli, Biochem. Biophys. Acta 30, 636–637.PubMedCrossRefGoogle Scholar
  32. Holliday, R., and Tarrant, G.M. (1972) Altered enzyme in ageing human fibroblasts, Nature 238, 26–30.PubMedCrossRefGoogle Scholar
  33. Holmes, A.M., Hesslewood, I.P., and Johnston, I.R. (1976) Evidence that DNA polymerase of calf thymus contains a subunit of molecular weight 155,000, Eur. J. Biochem. 62, 229–235.PubMedCrossRefGoogle Scholar
  34. Keijzer, W., Jaspers, N.G.J., Abrahams, P.J., Taylor, A.M.R., Arlett, C.F., Zelle, B., Takebe, H., Kinmont, P.D.S., and Bootsma, D. (1979) A seventh complementation group in excision-deficient xeroderma pigmentosum, Mutation Res. 62, 183–190.PubMedCrossRefGoogle Scholar
  35. Kuhnlein, U., Penhoet, E.E., and Linn, S. (1976) An altered apurinic DNA endonuclease activity in group A and group D xeroderma pigmentosum fibroblasts, Proc. Nat. Acad. Sci. USA 73, 1169–1173.PubMedCrossRefGoogle Scholar
  36. Laskowski, M. Sr. (1971) Deoxyribonuclease I, in “The Enzymes” (P.D. Boyer, ed.), vol. 4, pp. 289–311, Academic Press, New York.Google Scholar
  37. Latarjet, R. (1972) Interaction of radiation energy with nucleic acids, Current Topics Radiat. Res. Quart. 8, 1–38.Google Scholar
  38. Lawley, P.D. (1975) Excision of bases from DNA methylated by carcinogens in vivo and its possible significance in mutagenesis and carcinogenesis, in “Molecular Mechanisms for Repair of DNA” (P.C. Hanawalt, and R.B. Setlow, eds.) pp. 25–28, Plenum Press, New York.Google Scholar
  39. Lehman, I.R. (1974) DNA ligase: structure, mechanism, and function, Science 186, 790–797.PubMedCrossRefGoogle Scholar
  40. Lehmann, A.R. (1975) Postreplication repair of DNA in UV-irradiated mammalian cells, in “Molecular Mechanisms for Repair of DNA” (P.C. Hanawalt, and R.B. Setlow, eds.) pp. 617–623, Plenum Press, New York.Google Scholar
  41. Lehmann, A.R. (1972) Postreplication repair of DNA in ultraviolet-irradiated mammalian cells, J. Mol. Biol. 66, 319–337.PubMedCrossRefGoogle Scholar
  42. Lett, J.T., Caldwell, I., Dean, C.J., and Alexander, P. (1967) Rejoining of X-ray induced breaks in the DNA of leukaemia cells, Nature 214, 790–792.PubMedCrossRefGoogle Scholar
  43. Lindahl, T., and Nyberg, B. (1972) Rate of depurination of native deoxyribonucleic acid, Biochemistry 11, 3610–3618.PubMedCrossRefGoogle Scholar
  44. Lindahl, T., and Nyberg, B. (1974) Heat-induced deamination of cytosine residues in deoxyribonucleic acid, Biochemistry 13, 3405–3410.PubMedCrossRefGoogle Scholar
  45. Lindahl, T. (1979) DNA glycosylases, endonucleases for apurinic/ apyrimidinic sites and base excision-repair, Progr. Nucl. Acid Res. Mol. Biol. 22, 135–192.CrossRefGoogle Scholar
  46. Lindahl, T. (1976) New class of enzymes acting on damaged DNA, Nature 259, 64–66.PubMedCrossRefGoogle Scholar
  47. Lindahl, T., Ljungquist, S., Siegert, W., Nyberg, B., and Sperens, B. (1977) DNA N-glycosydases. Properties of uracil-DNA glycosydase from Escherichia coli, J. Biol. Chem. 252, 3286–3294.PubMedGoogle Scholar
  48. Lindahl, T., Gally, J.A., and Edelman, G.M. (1969a) Properties of deoxyribonuclease III from mammalian tissues, J. Biol. Chem. 244, 5014–5019.PubMedGoogle Scholar
  49. Lindahl, T., Gally, J.A., and Edelman, G.M. (1969b) Deoxyribonuclease IV: a new endonuclease from mammalian tissues, Proc. Nat. Acad. Sci. USA 62, 597–603.PubMedCrossRefGoogle Scholar
  50. Ljungquist, S., and Lindahl, T. (1974a) A mammalian endonuclease specific for apurinic sites in double-stranded deoxyribonucleic acid. I. Purification and general properties, J. Biol. Chem. 249, 1530–1535.PubMedGoogle Scholar
  51. Ljungquist, S., Andersson, A., and Lindahl, T. (1974b) A mammalian endonuclease specific for apurinic sites in double-stranded deoxyribonucleic acid. II. Further studies on the substrate specificity, J. Biol. Chem. 249, 1536–1540.PubMedGoogle Scholar
  52. Marmur, J., Anderson W.A., Matthews, L., Berns, K., Gajewska, E., Lane, D., and Doty, P. (1961) The effects of ultraviolet light on the biological and physical chemical properties of deoxyribonucleic acids, J. Cell Comp. Phys. 58, Suppl. 1, 33–55.CrossRefGoogle Scholar
  53. Mathelet, M., Clerici, L., Campagnari, F., and Talpaert-Borlé, M. (1978) The activity of mammalian polynucleotide ligase on X-irradiated DNAs, Biochem. Biophys. Acta 518, 138–149.PubMedGoogle Scholar
  54. Mattern, M., Hariharan, P.V., Dunlap, B.E., and Cerutti, P.A. (1973) DNA degradation and excision repair in γ-irradiated Chinese hamster ovary cells, Nature New Biol. 245, 230–232.PubMedGoogle Scholar
  55. McKune, K., and Holmes, A.M. (1979) Further studies on partially purified calf thymus DNA polymerase, Nucl. Acids Res. 6, 3341–3352.PubMedCrossRefGoogle Scholar
  56. Miller, E.C., and Miller, J.A. (1966) Mechanisms of chemical carcinogens: nature of proximate carcinogens and interactions with macromolecules, Pharmac. Rev. 18, 806–838.Google Scholar
  57. Miller, J.A. (1970) Carcinogenesis by chemicals: an overview, Cancer Res. 30, 559–576.PubMedGoogle Scholar
  58. Momparler, R.L., Rossi, M., and Labitan, A. (1973) Partial purification and properties of two forms of deoxyribonucleic acid polymerase from calf thymus, J. Biol. Chem. 248, 285–293.PubMedGoogle Scholar
  59. Murphy, T.M. (1974) Nucleic acids: interaction with solar U.V. radiation, Current Topics Radiat. Res. Quart. 10, 199–228.Google Scholar
  60. Radman, M. (1974) Phenomenology of an inducible mutagenic DNA repair pathway in Escherichia coli: SOS repair hypothesis, in “Molecular and Environmental Aspects of Mutagenesis” (L. Prakash, F. Sherman, M.W. Miller, C.W. Lawrence, and H.W. Taber, eds.) pp. 128–140, C. Thomas Publ., Inc., Springfield.Google Scholar
  61. Regan, J.D., Trosko, J.E., and Carrier, W.L. (1968) Evidence for excision of ultraviolet-induced pyrimidine dimers from the DNA of human cells in vitro, Biophys. J. 8, 319–325.PubMedCrossRefGoogle Scholar
  62. Regan, J.D., Setlow, R.B., and Ley, R.D. (1971) Normal and defective repair of damaged DNA in human cells; a sensitive assay utilizing the photolysis of bromodeoxyuridine, Proc. Nat. Acad. Sci. USA 68, 708–712.PubMedCrossRefGoogle Scholar
  63. Roth, H.D., and Lamola, A.A. (1972) Cleavage of thymine dimers sensitized by quinones. Chemically induced dynamic nuclear polarization in radical ions, J. Am. Chem. Soc. 94, 1013–1014.PubMedCrossRefGoogle Scholar
  64. Rupp, W., Wilde, C., Reno, D., and Howard-Flanders, P. (1971) Exchanges between DNA strands in ultraviolet irradiated E. coli, J. Mol. Biol. 31, 291–304.CrossRefGoogle Scholar
  65. Setlow, R.B., and Carrier, W.L. (1964) The disappearance of thymine dimers from DNA: an error-correcting mechanism, Proc. Nat. Acad. Sci. USA 51, 226–231.PubMedCrossRefGoogle Scholar
  66. Slor, H., and Lev, T. (1971) Acid deoxyribonuclease activity in purified calf thymus nuclei, Biochem. J. 123, 993–995.PubMedGoogle Scholar
  67. Smerdon, M.J., Tisty, T.D., and Lieberman, M.W. (1978) Distribution of ultraviolet-induced DNA repair synthesis in nuclease sensitive and resistant regions of human chromatin, Biochemistry 17, 2377–2386.PubMedCrossRefGoogle Scholar
  68. Söderhall, S., and Lindahl, T. (1975) Mammalian DNA ligase. Serological evidence for two separate enzymes, J. Biol. Chem. 250, 8438–8444.PubMedGoogle Scholar
  69. Söderhall, S., and Lindahl, T. (1976) DNA ligases of eukaryotes, FEBS Letters 67, 1–8.PubMedCrossRefGoogle Scholar
  70. Strauss, B.S. (1976) Repair of DNA adducts produced by alkylation, in “Ageing, Carcinogenesis, and Radiation Biology” (K.C. Smith, ed.) pp. 287–314, Plenum Press, New York.Google Scholar
  71. Sutherland, B.M., Runge, P., and Sutherland, J.C. (1974) DNA photoreactivating enzyme from placental mammals: origin and characteristics, Biochemistry 13, 4710–4714.PubMedCrossRefGoogle Scholar
  72. Takeshita, M., Grollman, A.P., Ohtsubo, E., and Ohtsubo, H. (1978) Interaction of bleomycin with DNA, Proc. Nat. Acad. Sci. USA 75, 5983–5987.PubMedCrossRefGoogle Scholar
  73. Talpaert-Borlé, M., Clerici, L., and Campagnari, F. (1979) Isolation and characterization of a uracil-DNA glycosylase from calf thymus, J. Biol. Chem. 254, 6387–6391.PubMedGoogle Scholar
  74. Wang, E., and Furth, J.J. (1977) Mammalian endonuclease, DNase V. Purification and properties of enzyme of calf thymus, J. Biol. Chem. 252, 116–124.PubMedGoogle Scholar
  75. Weiss, B. (1976) Endonuclease II of Escherichia coli is Exonuclease III*, J. Biol. Chem. 251, 1896–1901.PubMedGoogle Scholar
  76. Yoneda, M., and Bollum, F. (1965) Deoxynucleotide-polymerizing enzymes of calf thymus glands. I. Large scale purification of terminal and replicative deoxynucleotide transferase, J. Biol. Chem. 240, 3385–3391.PubMedGoogle Scholar
  77. Yoshida, S., Kondo, T., and Ando, T. (1974) Multiple molecular species of cytoplasmic DNA polymerase from calf thymus, Biochem. Biophys. Acta 353, 463–474.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1980

Authors and Affiliations

  • Francesco Campagnari
    • 1
  1. 1.Laboratory of BiochemistryBiology Group Ispra, D.G.XII, C.E.C., Joint Research CentreIspra (Va)Italy

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