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Studies on the Purification of Aspergillus niger Endopolygalacturonase by Agarose Gel Chromatography

  • J. F. Thibault
  • C. Mercier

Abstract

Endopolygalacturonase (endo PG) (poly (1,4-D-galacturonide) glycanohydrolase, E.C. 3.2.1.15), a pectolytic enzyme hydrolyzing randomly glycosidic linkages of pectic substances, can be purified by the usual methods. Nevertheless some original purification procedures have been developed using affinity chromatography on cross-linked pectic acid (1) or chromatography on agarose gel (2). On agarose it was observed that purified endo PG was delayed. In view of the resemblance between the anhydrogalactose residues of agarose and the anhydrogalacturonic ones of endo PG substrates, these workers suggested that biospecific interactions between agarose and endo PG were occurring. We used this suggestion to develop a method to purify endo PG from commercial Pectinase produced by Aspergillus niger and obtained from the Rapidase Company.

Keywords

Aspergillus Niger Glycosidic Linkage Pectic Substance Pectic Acid Pectolytic Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    REXOVA-BENKOVA, L. & TIBENSKY, V. Bio chim. ßiophys. Acta 268:187, 1972.Google Scholar
  2. 2.
    ENGLISH, P.D., MAGLOTHIN, A. & KEEGSTRA, K. Ptant Phystiot. 49: 293, 1972.Google Scholar
  3. 3.
    CRONE, H.D. J. Chnoma.tog 92: 1 27, 1974.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • J. F. Thibault
    • 1
  • C. Mercier
    • 1
  1. 1.Laboratoire De Biochimie Des AlimentsI.N.R.A.NantesFrance

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