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Physical-Chemical Factors Influencing the Resolution in Affinity Chromatography

  • V. Kasche
  • D. Gabel
  • H. Amneus

Abstract

When affinity chromatography is used for analytical or preparative purposes, such as for the separation of modified enzyme molecules from native enzymes, a biospecific adsorbent with a high resolution is required. The resolution is determined by the following physical-chemical factors: (a) distribution coefficient in the sorption chromatographic procedure, (b) specificity of the binding between adsorbent and enzyme and its modulation by desorbing ligands, and (c) kinetics of the adsorption and desorption. The influence of (a) and (b) on the resolution has been reported previously (1–3). The kinetics of adsorption and desorption are reported here. The number of adsorbent molecules, determined by stationary methods, is generally higher than the corresponding number determined by dynamic methods (loading a chromatographic column).

Keywords

Distribution Coefficient Steric Hindrance Affinity Chromatography Dynamic Method Adsorbent Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    KASCHE, V. Biophys. Res.Comm. 38: 875, 1970.Google Scholar
  2. 2.
    KASCHE, V. Studia biophysica 35: 45, 1973.Google Scholar
  3. 3.
    AMNEUS, H., GABEL, D., & KASCHE, V. J. Chtomauogk. 120: 391, 1976.Google Scholar
  4. 4.
    KASCHE, V., AMNEUS, H., GABEL, D., & NASLUND, L. Biochim. BLopho. Acta 490:1, 1977.Google Scholar
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    KASCHE, V. Rad.Loanat. Letten: 3: 51, 1970.Google Scholar
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    KASCHE, V. Arsch. Blochem. BLophye. 173: 269, 1976.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • V. Kasche
    • 1
  • D. Gabel
    • 2
  • H. Amneus
    • 3
  1. 1.Biology and Departments University of BremenBremenFederal Republic Germany
  2. 2.Chemistry Departments University of BremenBremenFederal Republic Germany
  3. 3.Department of Physical BiologyUppsala UniversityUppsalaSweden

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