Physical-Chemical Factors Influencing the Resolution in Affinity Chromatography

  • V. Kasche
  • D. Gabel
  • H. Amneus


When affinity chromatography is used for analytical or preparative purposes, such as for the separation of modified enzyme molecules from native enzymes, a biospecific adsorbent with a high resolution is required. The resolution is determined by the following physical-chemical factors: (a) distribution coefficient in the sorption chromatographic procedure, (b) specificity of the binding between adsorbent and enzyme and its modulation by desorbing ligands, and (c) kinetics of the adsorption and desorption. The influence of (a) and (b) on the resolution has been reported previously (1–3). The kinetics of adsorption and desorption are reported here. The number of adsorbent molecules, determined by stationary methods, is generally higher than the corresponding number determined by dynamic methods (loading a chromatographic column).


Distribution Coefficient Steric Hindrance Affinity Chromatography Dynamic Method Adsorbent Molecule 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • V. Kasche
    • 1
  • D. Gabel
    • 2
  • H. Amneus
    • 3
  1. 1.Biology and Departments University of BremenBremenFederal Republic Germany
  2. 2.Chemistry Departments University of BremenBremenFederal Republic Germany
  3. 3.Department of Physical BiologyUppsala UniversityUppsalaSweden

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