Guanosine Nucleotide Analogues as General Ligands in Affinity Chromatography
The development in recent years of biospecific adsorbents of the general ligand type used in affinity chromatography has proven most valuable. The most widely used ligands for enzyme purification have been analogues of adenosine mono- and dinucleotides (1–5). We now report on the synthesis of a new guanosine nucleotide analogue, 8(6-aminohexyl)-amino-GMP and its utilization for the purification of IMP dehydrogenase from Aerobacter aerogenes.
KeywordsAffinity Chromatography Ammonium Sulfate Enzyme Purification Crude Reaction Mixture General Ligand
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