Guanosine Nucleotide Analogues as General Ligands in Affinity Chromatography

  • Peter E. Brodelius
  • Nathan O. Kaplan


The development in recent years of biospecific adsorbents of the general ligand type used in affinity chromatography has proven most valuable. The most widely used ligands for enzyme purification have been analogues of adenosine mono- and dinucleotides (1–5). We now report on the synthesis of a new guanosine nucleotide analogue, 8(6-aminohexyl)-amino-GMP and its utilization for the purification of IMP dehydrogenase from Aerobacter aerogenes.


Affinity Chromatography Ammonium Sulfate Enzyme Purification Crude Reaction Mixture General Ligand 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Peter E. Brodelius
    • 1
  • Nathan O. Kaplan
    • 1
  1. 1.Department of ChemistryUniversity of California at San DiegoLa JollaUSA

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