Purification and Immobilization of a Fungal ß-Galactosidase (Lactase)

  • J. L. Leuba
  • F. Widmer
  • D. Magnolato


Several glycosidases secreted by the fungus Aspehgillus nigen have recently been purified and characterized (1-5). These enzymes were found to be glycoproteins; and their pH optima ranged from 3.5 to 5.0. We now report the purification of the A. nigen ß-galactosidase from a commercial preparation. The occur-rance of this enzyme in multiple molecular forms has been suggested by earlier work (6). The aim of this study was to achieve separation and characterization of these isoenzymes.


Glutaraldehyde Solution Lactose Hydrolysis Chitosan Particle Lactose Solution Acidic Whey 
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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • J. L. Leuba
    • 1
  • F. Widmer
    • 1
  • D. Magnolato
    • 1
  1. 1.Nestle Products Technical Assistance Co. Ltd.Research Department La Tour-de-PeilzSwitzerland

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