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Principles of Multi-Enzyme Purification by Affinity Chromatography

  • C.-Y. Lee
  • A. Leigh-Brown
  • C. Langley
  • B. Pegoraro
  • J. Lopez-Barea
  • D. Charles

Abstract

Following our previous reports on the use of 8-substituted adenine nucleotide derivatives as general ligands for affinity chromatography (1–3), several important new principles have been developed regarding multi-enzyme purifications from crude extracts by general ligand affinity chromatography.

Keywords

Affinity Chromatography Pyruvate Kinase Malate Dehydrogenase Affinity Column Malic Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    LEE, C-Y., LAPPI, D.A., WERMUTH, B., EVERSE, J. & KAPLAN, N.O.: Anch. Biochem. Biophys. 163: 561, 1974.CrossRefGoogle Scholar
  2. 2.
    LEE, C-Y. & JOHANSSON, L-J.: Anat. Biochem. 77: 90, 1977.CrossRefGoogle Scholar
  3. 3.
    LEE, C-Y., LAZARUS, L.H., KABAKOFF, D.S., LAVER, M.B., RUSSELL, P.J. & KAPLAN, N.O.: Anch Biochem. Biophys. 178: 8, 1977.Google Scholar
  4. 4.
    PEGORARO, B. & LEE, C-Y.: Biocheen. Biophy. Acta, in press.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • C.-Y. Lee
    • 1
  • A. Leigh-Brown
    • 1
  • C. Langley
    • 1
  • B. Pegoraro
    • 1
  • J. Lopez-Barea
    • 1
  • D. Charles
    • 1
  1. 1.Laboratory of Environmental MutagenesisNational Institute of Environmental Health SciencesUSA

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