Pressure-Driven Affinity Sorption of Trypsin Inhibitors

Abstract

The enzyme-coupled ultrafiltration membrane system described by Gregor and Rauf (1) is characterized by a very high loading of bound enzyme, with the specific activity of the native enzyme largely retained. The system has unique advantages. Since the feed streams are pumped rapidly under pressure, the contact times are extremely short, such that diffusion control of the process is virtually eliminated. The nature of the membrane system lends itself to the use of high speed, repetitive processing.