Pressure-Driven Affinity Sorption of Trypsin Inhibitors

  • Harry P. Gregor
  • Paul W. Rauf


The enzyme-coupled ultrafiltration membrane system described by Gregor and Rauf (1) is characterized by a very high loading of bound enzyme, with the specific activity of the native enzyme largely retained. The system has unique advantages. Since the feed streams are pumped rapidly under pressure, the contact times are extremely short, such that diffusion control of the process is virtually eliminated. The nature of the membrane system lends itself to the use of high speed, repetitive processing.


Feed Stream Ultrafiltration Membrane Cyanogen Bromide Repetitive Processing Affinity Sorption 
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  1. 1.
    GREGOR, H.P. & RAUF, P.W. Biotechnol. Bioeng. 17: 445 1975.CrossRefGoogle Scholar
  2. 2.
    GRYTE, C.C. & GREGOR, H.P. J. Polymei Sci.. Physics. Ed. 14: 1839, 1855, 1976.CrossRefGoogle Scholar
  3. 3.
    RAUF, P.W., Ph.D. Thesis, Columbia University, 1974.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Harry P. Gregor
    • 1
  • Paul W. Rauf
    • 1
  1. 1.Department of Chemical Engineering and Applied ChemistryColumbia UniversityNew YorkUSA

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