Immobilization of Proteins on Polyhydrazides

  • Meir Wilchek
  • Talia Miron

Abstract

Immobilized proteins have been prepared by covalent coupling of proteins to various insoluble carriers. The supports used were polyamino acids, cellulose, polyacrylamide derivatives, beaded glass, maleic anhydride copolymers (EMA), dialdehyde starch, beaded agarose, crosslinked dextrans and nylons (1). Different reactions were used to couple the protein to the carriers, including the azide, carbodiimide, diazonium salt, acyl chloride, cyanogen bromide, isothiocyanate, anhydride and other methods (1).

Keywords

Cellulose Amide Polysaccharide Immobilization Trypsin 

References

  1. 1.
    Zaborsky, O. “Immobilized Enzymes,” CRC Press, Cleveland, 1973.Google Scholar
  2. 2.
    Miron, T., Carter, W.G., & Wilchek, M. J. Soti d Phase B-.achem. 1: 225, 1976.Google Scholar
  3. 3.
    Morris, D.L., Campbell, J., & Hornby, W.E. B-.achem. J. 147: 593, 1975.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Meir Wilchek
    • 1
  • Talia Miron
    • 1
  1. 1.Department of BiophysicsThe Weizmann Institute of Science RehovotIsrael

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