Enzyme Activity at a Gas Solid Interphase: Oxidation of Methanol to Formaldehyde
Methanol-consuming yeasts have an FAD-dependent methanol oxidase, which catalyzes the reaction between methanol and oxygen to form formaldehyde and hydrogen peroxide. The catalâse that is present in the same peroxisomes decomposes the hydrogen peroxide; it also acts as a formaldehyde producer by oxidation of the methanol (1,2). A subsequent enzymatic step incorporates formaldehyde into different metabolic pathways through an NADH2 - dependent formaldehyde dehydrogenase, which requires a mechanism for NAD regeneration. Therefore, non-viable cells will not decompose formaldehyde since the NAD cannot be regenerated (1).
KeywordsMethanol Oxida Methanol Vapor Methanol Feed Exceptional Stability Initial Reaction Velocity
Unable to display preview. Download preview PDF.
- 1.KATO, N., TANI, Y., & OGATA, K. Agn. Biol. Chem. 38: 675, 1974.Google Scholar
- 2.SABAIO, F., ATSUO, T., SUSUMA, K., SHIGEKI, Y., YUTAKA, T., & MASAKO, O. J. Bacteniol. 123: 317, 1975.Google Scholar
- 3.ZUNIGA, V. M.S. Thesis, CIEA del IPN; Mexico, 1977.Google Scholar
- 4.SAHM, H., B. DIX. L. EGGELING & R. ROGGENKAMP. Abst. 5th Intenn. Fenment. Symp., Berlin, 1976.Google Scholar
- 5.WALKER, F. “Formaldehyde,” 3rd Edit., Reinhold, New York, 1966, p. 469.Google Scholar
- 6.VOGEL, A.I. “A Textbook of Practical Organic Chemistry Including Qualitative Organic Analysis,” 3rd Edit., Longmans, New York, 1962, p. 325.Google Scholar
- 7.HELMKAMP, H. “Sinopsis de Quimica Organica,” 2nd Edit., McGraw-Hill, New York, 1968, p. 68.Google Scholar