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Continuous Measurements on Immobilized Cells by a Mass Filter

  • J. C. Weaver
  • F. M. Reames
  • L. DeAlleaume
  • C. R. Perley
  • C. L. Cooney

Abstract

The possibility of a continuous assay based on direct measurement of volatile reactants of an enzyme-catalyzed reaction has been described (1,2). Extension of this analytical approach to immobilized cells should offer the same potential for speed and sensitivity. Also, it should allow a variety of species, such as carbon dioxide, HCHO, methanol, or acetic acid, to be monitored directly. Or, by downstream continuous enzymatic conversion, using a suitabale immobilized enzyme, nonvolatile species, such as glucose, pyruvate, lactate, and acetylcholine, could be used. The rationale for pursuing such measurements includes the possibility of studying the effects of many stimuli on immobilized cells. It also includes applications directed at environmental measurements, including the possibility of continuous, rapidly responding bioassays for environmental monitoring.

Keywords

Immobilize Cell Module Depth Environmental Measurement Reaction Module Mass Filter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    WEAVER, J.C., MASON, M.K., JARRELL, J.A., & PETERSON, J.W. Biochim. Biophys. Acta. 438: 296, 1976.CrossRefGoogle Scholar
  2. 2.
    WEAVER, J.C. in “Biomedical Applications of Immobilized Enzymes and Proteins,” vol. 2 (T.M.S. Chang, ed.), Plenum, New York, 1977, p. 207.Google Scholar
  3. 3.
    MATTIASSON, B., LARSON, P.-D., & MOSBACH, K. Nature 268: 519, 1977.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • J. C. Weaver
    • 1
  • F. M. Reames
    • 1
  • L. DeAlleaume
    • 1
  • C. R. Perley
    • 1
  • C. L. Cooney
    • 1
  1. 1.Massachusetts Institute of TechnologyCambridgeUSA

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