Continuous Measurements on Immobilized Cells by a Mass Filter
The possibility of a continuous assay based on direct measurement of volatile reactants of an enzyme-catalyzed reaction has been described (1,2). Extension of this analytical approach to immobilized cells should offer the same potential for speed and sensitivity. Also, it should allow a variety of species, such as carbon dioxide, HCHO, methanol, or acetic acid, to be monitored directly. Or, by downstream continuous enzymatic conversion, using a suitabale immobilized enzyme, nonvolatile species, such as glucose, pyruvate, lactate, and acetylcholine, could be used. The rationale for pursuing such measurements includes the possibility of studying the effects of many stimuli on immobilized cells. It also includes applications directed at environmental measurements, including the possibility of continuous, rapidly responding bioassays for environmental monitoring.
KeywordsImmobilize Cell Module Depth Environmental Measurement Reaction Module Mass Filter
- 2.WEAVER, J.C. in “Biomedical Applications of Immobilized Enzymes and Proteins,” vol. 2 (T.M.S. Chang, ed.), Plenum, New York, 1977, p. 207.Google Scholar