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Procedure for the Simultaneous Large-Scale Isolation of Pullulanase and 1,4-α-Glucan Phosphorylase Involving Liquid-Liquid Separations

  • H. Hustedt
  • K. H. Kroner
  • M.-R. Kula

Abstract

In enzyme isolation procedures liquid-liquid partition can be employed using aqueous two-phase systems, based on two hydrophylic polymers like polyethyleneglycol (PEG) and dextran or on a suitable polymer and certain salts (PEG and phosphate) (1). We have used such a two-phase systems for the simultaneous isolation of pullulanase (2) and 1,4-α-glucan phosphorylase (3) from Klebsiella pneumoniae.

Keywords

Potassium Phosphate Potassium Chloride Ammonium Bromide Bottom Phase Sodium Cholate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    ALBERTSSON, P.A. “Partition of CelJ Particles and Makromolekules,” Wiley, New York, 1971.Google Scholar
  2. 2.
    BENDER, H. Aich. Miciobiol. 71: 331, 1970.Google Scholar
  3. 3.
    LINDER, D., KURZ, K., BENDER, H. & WALLENFELS, K. Eun. J. Biochem. 70: 291, 1976.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • H. Hustedt
    • 1
  • K. H. Kroner
    • 1
  • M.-R. Kula
    • 1
  1. 1.Gesellschaft fur Biotechnologische Forschung mbH.Braunschweig-StockheimFederal Republic Germany

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