Procedure for the Simultaneous Large-Scale Isolation of Pullulanase and 1,4-α-Glucan Phosphorylase Involving Liquid-Liquid Separations
In enzyme isolation procedures liquid-liquid partition can be employed using aqueous two-phase systems, based on two hydrophylic polymers like polyethyleneglycol (PEG) and dextran or on a suitable polymer and certain salts (PEG and phosphate) (1). We have used such a two-phase systems for the simultaneous isolation of pullulanase (2) and 1,4-α-glucan phosphorylase (3) from Klebsiella pneumoniae.
KeywordsPotassium Phosphate Potassium Chloride Ammonium Bromide Bottom Phase Sodium Cholate
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ALBERTSSON, P.A. “Partition of CelJ Particles and Makromolekules,” Wiley, New York, 1971.Google Scholar
BENDER, H. Aich. Miciobiol.
: 331, 1970.Google Scholar
LINDER, D., KURZ, K., BENDER, H. & WALLENFELS, K. Eun. J. Biochem
: 291, 1976.Google Scholar
© Plenum Press, New York 1978