On the Mechanism of Protein Cross-Linking with Glutaral-Dehyde

  • Sven Branner-Jorgensen


Four theories have been developed to explain the cross-linking reaction of glutaraldehyde with proteins: (a) simple Schiff-base formation; (b) reaction of glutaraldehyde pplymers to form Michael-type additions to the protein (1); (c) glutaraldehyde polymers forming conjugation stabilized Schiff-bases with the protein (2); and (d) pyridinium ion theory (3). We have tested these different mechanistic proposals with the following dialdehydes: succinic, glutaric, 2,4-dimethylglutaric (2,4-DMGA), and adipic.


Mechanistic Proposal Reactive Reagent Imine Bond Unsaturated Polymer Suitable Chain 
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    RICHARDS, F.M. & KNOWLES, J.R. J. Mat. Biot. 37: 231, 1968.Google Scholar
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    MONSAN, P., PUZO, G. & MAZARGUIL, H. Biochemie 57: 1281, 1975.CrossRefGoogle Scholar
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    HARDY, P.M., NICHOLLS, A.C. & RYDON, H.N. J. Chem. Soc. Perkin 1 1976: 958.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Sven Branner-Jorgensen
    • 1
  1. 1.NOVO Industri A/S BagsvaerdDenmark

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