Advertisement

On the Mechanism of Protein Cross-Linking with Glutaral-Dehyde

  • Sven Branner-Jorgensen

Abstract

Four theories have been developed to explain the cross-linking reaction of glutaraldehyde with proteins: (a) simple Schiff-base formation; (b) reaction of glutaraldehyde pplymers to form Michael-type additions to the protein (1); (c) glutaraldehyde polymers forming conjugation stabilized Schiff-bases with the protein (2); and (d) pyridinium ion theory (3). We have tested these different mechanistic proposals with the following dialdehydes: succinic, glutaric, 2,4-dimethylglutaric (2,4-DMGA), and adipic.

Keywords

Mechanistic Proposal Reactive Reagent Imine Bond Unsaturated Polymer Suitable Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    RICHARDS, F.M. & KNOWLES, J.R. J. Mat. Biot. 37: 231, 1968.Google Scholar
  2. 2.
    MONSAN, P., PUZO, G. & MAZARGUIL, H. Biochemie 57: 1281, 1975.CrossRefGoogle Scholar
  3. 3.
    HARDY, P.M., NICHOLLS, A.C. & RYDON, H.N. J. Chem. Soc. Perkin 1 1976: 958.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Sven Branner-Jorgensen
    • 1
  1. 1.NOVO Industri A/S BagsvaerdDenmark

Personalised recommendations