Studies of Hemoglobin and Allosteric Enzymes Under Artificial Conformational Constraints
The concept of a conformational change of regulatory enzyme is not well-defined; and the study of the effect of artificial conformational constraints on enzyme behavior is of interest. In accord with Koshland (1), it seems reasonable to attempt to “freeze” some of the conformational states, possibly by tight chemical immobilization of the regulatory enzymes inside an artificial membrane. The hemoglobin modification of conformation is generally discussed as a general model for allosteric enzymes, and it is of interest to compare the relative effect of immobilization on an allosteric enzyme and on hemoglobin.
KeywordsDiffusional Limitation Pyruvate Kinase Regulatory Enzyme Sodium Dithionite Oxygen Binding
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