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Cellulose Bead Entrapped Whole Cell Glucose Isomerase in Fructose Syrup Production

  • Yu-Yen Linko
  • R. Viskari
  • L. Pohjola
  • P. Linko

Abstract

Purified and whole cell glucose isomerase (GI) has been immobilized on various supports (1). Most research has involved Baciteus and Stnep-tomyces enzymes. We have developed a simple and mild technique to entrap whole cell Actinop1anea m-.44ounien4-L4 GI in cellulose (2,3).The method involves suspending dried cells in a-cellulose, dissolved in a melt of N-ethyl-pyridinium chloride (NEPC) and dimethylformamide (DMF). The GI cellulose could be regenerated in fiber or bead form by passing into water through an orifice of dia 0.8 mm, treating with glutaraldehyde for crosslinking, and vacuum drying to about 24% dry weight. This is believed to be the first time an enzyme has been immobilized within regenerated pure cellulose beads.

Keywords

Pressure Drop Scan Electron Micrographs Glucose Solution Pure Cellulose External Diffusion 
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References

  1. 1.
    CASEY, J.P. Die Stanke 29:196, 1977.CrossRefGoogle Scholar
  2. 2.
    LINKO, Y.—Y., POHJOLA, L., VISKARI, R. & LINKO, M. FEBS Lett. 62:77, 1976.Google Scholar
  3. 3.
    LINKO, Y.—Y., POHJOLA, L. & LINKO, P. P4oce4d Bio-chemibtny 12: 14, 1977.Google Scholar
  4. 4.
    CHEN, L.F. & TSAO, G.T. Btiotechno4. Bioeng. 18: 1507, 1976.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Yu-Yen Linko
    • 1
  • R. Viskari
    • 2
  • L. Pohjola
    • 2
  • P. Linko
    • 2
  1. 1.Academy of FinlandEspooFinland
  2. 2.Department of Chemistry HelsinkiUniversity of TechnologyEspooFinland

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