Benzene Metabolism by Bacterial Cells Immobilized in Polyacrylamide Gel
Many micro-organisms can oxygenate hydrocarbons under milder conditions than required for chemical oxygenation. These reactions provide an interesting set of model conversions in which to compare chemical with biological conversion. However, one of the greatest drawbacks with biological catalytic systems is their relative instability. The present work was carried out using a strain of Pseudomonas putida to evaluate whether the hydrocarbon-oxidizing system could be stabilized, and to investigate the potential for reactivation of the system after loss of activity.