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Immobilization of Enzymes, Microbial Cells, and Organelles by Inclusion with Photo-Crosslinkable Resins

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Enzyme Engineering

Abstract

This communication deals with the application of photo-crosslinkable resins to inclusion of enzymes, microbial cells and organelles. The method consists of mixing (a) liquid oligomers of suitable photo-crosslinkable resin(s) containing photo-sensitive functional groups, (b) an appropriate initiator, and (c) an enzyme solution or suspension of cells or organelles, followed by illumination with near-ultraviolet light for only a few minutes. This simple and convenient procedure produces tailor-made matrices in which biologically active macromolecules are successfully entrapped.

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References

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Author information

Authors and Affiliations

  1. Laboratory of Industrial Biochemistry, Faculty of Engineering Kyoto University, Kyoto, Japan

    Saburo Fukui, Atsuo Tanaka & Gerald Gellf

Authors
  1. Saburo Fukui
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  2. Atsuo Tanaka
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  3. Gerald Gellf
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Editor information

Editors and Affiliations

  1. Départment de Génie Biologique, Université de Technologie, Compiègne, France

    Georges B. Broun

  2. Institut für Organische Chemie, Freie Universität Berlin, Berlin, Federal Republic of Germany

    Georg Manecke

  3. Department of Pharmacology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

    Lemuel B. Wingard Jr.

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© 1978 Plenum Press, New York

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Fukui, S., Tanaka, A., Gellf, G. (1978). Immobilization of Enzymes, Microbial Cells, and Organelles by Inclusion with Photo-Crosslinkable Resins. In: Broun, G.B., Manecke, G., Wingard, L.B. (eds) Enzyme Engineering. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6985-1_61

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  • DOI: https://doi.org/10.1007/978-1-4684-6985-1_61

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-6987-5

  • Online ISBN: 978-1-4684-6985-1

  • eBook Packages: Springer Book Archive

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