Properties of Heat Stable Enzymes of Extreme Thermophiles
Thermophilic organisms have become the subject of intense research interest in the recent decade. Biochemists are interested in molecular mechanisms of thermophilly and of biological adaptation to high temperatures (1). Thermophiles also are useful materials for the study of such biochemical reactions as protein biosynthesis (2), nucleic acid synthesis (3), and oxidative phosphorylation (4) in which components from mesophilic sources are so labile that the elucidation of reaction properties is often difficult. In addition, thermophiles are fascinating organisms for enzyme engineering since they are resistant to heat and other chemical denaturants. Also, insight into the molecular basis of unusual stabilities of these enzymes will make it possible to find out a new, effective way of chemical modification for making highly stable enzymes from unstable biocatalysts of mesophilic organisms.
KeywordsAmino Acid Composition Guanidine Hydrochloride Phosphoglycerate Kinase Nucleic Acid Synthesis Enzyme Engineering
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