Production of 6APA in the Penicillin G Fermentation Plant by Using Fiber-Entrapped Penicillin Amidase

  • F. Giacobbe
  • A. Iasonna
  • F. Cecer


In the last few years improvements in enzyme immobilization techniques have enabled enzymatic hydrolysis of penicillin G for the production of 6APA to become a viable alternative to chemical hydrolysis. This paper presents a novel process method for producing 6APA by enzymatic hydrolysis. In the overall process penicillin G is separated from the fermented broth by solvent extraction; the end product of the solvent extraction is generally a crude concentrated solution of penicillin G,K from which the penicillin is crystallized by azeotropic distillation with butanol. In the conventional 6APA enzymatic process the dried crystals of penicillin G are dissolved in water, and sent to the hydrolysis stage to yield 6APA and phenyl acetic acid. It would appear that if the 6APA plant was located adjacent to the penicillin fermentation plant, then it would be possible to hydrolyze directly the crude solution of penicillin G obtained from the solvent extraction, by-passing the crystallization stage. In this stage about 8–10% of the penicillin G is lost either in the mother liquor or through degradation during the azeotropic distillation. In addition it should also be possible to recover the phenyl acetic acid, which could be used as a precursor in the penicillin fermentation.


Mother Liquor Phenyl Acetic Acid Caustic Soda Azeotropic Distillation Fermented Broth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    DINELLI, D. Paocess Blochem. 7: 9, 1972.Google Scholar
  2. 2.
    BATCHELOR, F.R., CHAIN, HARDY, T.L. MANSFORD, E.B K.R.L., ROLINSON, G.H., Pnoc. Roy Soc. 554: 498, 1961.CrossRefGoogle Scholar
  3. 3.
    ALICINO, J.F. Anat. Chem. 33: 648, 1961.CrossRefGoogle Scholar
  4. 4.
    BOMSTEIN, J. Anat. Chem. 37: 576, 1965.CrossRefGoogle Scholar
  5. 5.
    BALASINGHAM, K., D., DUNNILL, P., LILLY, WARBURTON, M.D. Bloch. Bloph. Acta 276: 250, 1972.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • F. Giacobbe
    • 1
  • A. Iasonna
    • 1
  • F. Cecer
    • 2
  1. 1.Biochem Design S.p.A.RomaItaly
  2. 2.SNAM Progetti Microbiological LaboratoryMonterotondoItaly

Personalised recommendations