Regeneration of ATP by Immobilized Microbial Cells and Its Utilization for the Synthesis of Nucleotides
A number of biosynthetic methods for the production of various important nucleotides from their precursors using microbial cells have been developed in Japan (1); and some of these nucleotides have been produced industrially for more than a decade. For the production of those nucleotides an energy-rich phosphate compound, namely ATP, usually is required; and it is supplied to the reaction media either exogenously or by the naturally occurring ATP regeneration system involved in microbial cells. In the present paper the authors describe the immobilization of microbial cells and the utilization of such immobilized cells as the ATP regenerator for the continuous production of various nucleotides.
KeywordsMicrobial Cell Immobilize Cell Ammonium Persulfate Choline Chloride Molar Conversion
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