Immobilization of Enzyme on PMG and Its Application to the Urea Monitoring Apparatus
Poly-γ-methyl-L-glutamate (PMG) has a protein-like character (1,2) and easily gives various types of fiber or film. Also, it coats various surfaces of solid materials. PMG has the proper mechanical strength and hydrophilic features to be suitable as a matrix for enzyme immobilization. Entrapment of enzyme in PMG fiber has been tried (3); however, the chemical immobilization of enzyme on polyglutamate (PG) has not been reported yet.
KeywordsEnzyme Immobilization Hydrazine Hydrate Native Enzyme Blood Flow Rate Heat Stability
Unable to display preview. Download preview PDF.
- 1.BRESLOW, E., BEYCHOK, S., HARDMAN, K.D. & GURD, F.R.N. J. Biot. Chem 240:304, 1965.Google Scholar
- 3.DINELLI, D. Piocess Biochem. 7(8): 9, 1972.Google Scholar
- 4.YUGARI, Y. & MINAMOTO, Y. US Patent No. 3, 985, 617; 1976.Google Scholar
- 5.YUGARI, Y. et at.,to be published.Google Scholar
- 6.MINAMOTO, Y., KATO, N., KOMIYA, K., YUGARI, Y., NASUNO, S., SUZUKI, Y., & MIMURA, N. J. Japanese Medieat Instiuments. 46: suppl. 176, 1976.Google Scholar