Analytical Application of Collagen Enzymatic Membranes for a Glucose Sensor
The aim of this work was to test the analytical potentialities of our enzymatic collagen membranes combined with electrochemical detectors. Our glucose sensor is based on glucose oxidation by dissolved oxygen, as catalyzed by β-D-glucose oxidase (GOD, EC 18.104.22.168), to produce gluconic acid and hydrogen peroxide. The GOD is bound to the collagen film by the acyl-azide procedure (1–3). The hydrogen peroxide is detected by anodic oxidation on a platinum disk electrode at +650mV versus a Ag/AgC1 reference electrode. A differential method is used by comparison of the current output of two electrodes, one containing GOD(E1) and the other a non-enzymic membrane (E2).
KeywordsGlucose Oxidation Gluconic Acid Glucose Sensor Differential Method Collagen Film
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