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Cultural Conditions for the Microbial Production of β-Tyrosinase and Tryptophanase

  • H. Yamada
  • H. Kumagai
  • H. Enei
  • H. Nakazawa
  • K. Mitsugi

Abstract

β-Tyrosinase (tyrosine phenol-lyase: EC 4.1.99.2) and tryptophanse (tryptophan indole-lyase: EC 4.1.99.1) are enzymes which respectively catalyze the degradation of L-tyrosine and L-tryptophan, and require pyridoxal 5’-phosphate (PLP) as a cofactor. Crystalline preparations of these enzymes were prepared in our laboratories from Escherichia intermedia and Proteus rettgeri, and their properties were established in some detail. The crystalline enzymes were shown to catalyze a variety of α,β-elimination (Eq. 1), β-replacement (Eq. 2), and the reverse of α,β-elimination reactions (Eq. 3) (1–3).

Keywords

Succinic Acid Corn Steep Liquor Soybean Protein Lauryl Alcohol Sodium Sulfite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • H. Yamada
    • 1
  • H. Kumagai
    • 2
  • H. Enei
    • 3
  • H. Nakazawa
    • 3
  • K. Mitsugi
    • 3
  1. 1.Departments of Agricultural ChemistryKyoto UniversityKyotoJapan
  2. 2.Food Science and TechnologyKyoto UniversityKyotoJapan
  3. 3.Central Research LaboratoriesAjinomoto Co., Inc.KawasakiJapan

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