Galactose Conversion Using a Microcapsule Immobilized Multienzyme Cofactor Recycling System

  • T. M. S. Chang
  • N. Kuntarian


Immobilized enzymes, especially microencapsulated enzymes, have been used to replace enzyme deficiency in some forms of inborn errors of metabolism in animals (1,2). Still, the clinical use of immobilized enzymes for inborn errors of metabolism is not yet widely feasible. This is due partly to the fact that in most of the inborn errors of metabolism, complex multienzyme system with cofactor requirements are necessary. The further development of multienzyme systems with cofactor regeneration is thus essential (1,3). Basic studies carried out using microencapsulated multienzyme systems have demonstrated the feasibility of recycling cofactors like ATP, NAD and NADP (1,4,5). Galactosemia is usually due to a deficiency in galactose-1-P-uridyl-transferase or, less frequently, to a deficiency in galactokinase. Since galactokinase is more readily available, the present report is a feasibility study of microencapsulating this enzyme together with a cofactor recycling enzyme system for the conversion of galactose.


Immobilize Enzyme Pyruvate Kinase Phosphoenol Pyruvate Inborn Error Artificial Cell 
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    CHANG, T.M.S. “Biomedical Applications of Immobilized Enzymes and Proteins,” vol. 1, Plenum, New York, 1977, pp. 428, 2, 359.Google Scholar
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    CHANG, T.M.S. “Artificial Cells,” Charles C. Thomas Publisher, Springfield, Ill., 1972.Google Scholar
  3. CHANG, T.M.S. Meth. in Enzymotogy 44:999, 1976.Google Scholar
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    CAMPBELL, J. & CHANG, T.M.S. Biochim. Biophys. Acta. 397: 101, 1975.CrossRefGoogle Scholar
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    CAMPBELL, J. & CHANG, T.M.S. Biochem. Biophfs. Res. Commun. 69: 562, 1976.CrossRefGoogle Scholar
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    COUSINEAU, J. & CHANG, T.M.S. in “Artificial Kidney Artificial Liver and Artificial Cells,” (T.M.S. Chang, ed.), Plenum, New York, 1978, p. 302.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • T. M. S. Chang
    • 1
  • N. Kuntarian
    • 1
  1. 1.Department of PhysiologyMcGill UniversityMontrealCanada

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