It is well known (1) that one of the processes of inactivation of aminoglycosides by some bacteria is a structural modification of these antibiotics, e.g. N-acetylation, o-adenylylation, o-phosphorylation. The study of the structure activity relationship requires the preparation of specifically modified aminoglycosides at one of the amino or hydroxyl functions. The only way to obtain such specificity is by enzymatic reactions. We therefore used enzymes isolated from resisting bacterial strains, performing specific modification of aminoglycosides. We thus modified Gentamicin, Sisomicin and Tobramycin.
KeywordsBacterial Strain Enzymatic Reaction Structure Activity Relationship Specific Modification DEAE Cellulose
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