Immobilization of Hydrogenase from Alcaligenes Eutrophus H 16 on Glass Beads
This hydrogenase, a flavo-enzyme, has interesting properties. It transfers electrons directly to NAD+ and other artificial acceptors such as methylviologen or benzylviologen. Furthermore it is rather insensitive to oxygen (1). Therefore we immobilized the hydrogenase on a silanized CPG-10 carrier (48.6 nm, 120–200 mesh) by different coupling methods (2).
KeywordsGlass Bead Free Enzyme Coupling Method Maximal Specific Activity Photometric Assay
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