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Immobilization of Hydrogenase from Alcaligenes Eutrophus H 16 on Glass Beads

  • P. Egerer
  • E. Schleicher
  • H. Simon

Abstract

This hydrogenase, a flavo-enzyme, has interesting properties. It transfers electrons directly to NAD+ and other artificial acceptors such as methylviologen or benzylviologen. Furthermore it is rather insensitive to oxygen (1). Therefore we immobilized the hydrogenase on a silanized CPG-10 carrier (48.6 nm, 120–200 mesh) by different coupling methods (2).

Keywords

Glass Bead Free Enzyme Coupling Method Maximal Specific Activity Photometric Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    SCHNEIDER, K. & SCHLEGEL, H.G. Biochim. Biophys. Acta 452: 66, 1976.CrossRefGoogle Scholar
  2. 2.
    WEETALL, H.H. & FILBERT, A.M., Meth. in Enzymology 34:59, 1972.CrossRefGoogle Scholar
  3. 3.
    LAPPI, D.A., STOLZENBACH, F.E., KAPLAN, N.O. & KAMEN, M.D. Bioehem. Biophys. Res. Comm. 69: 878, 1976.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • P. Egerer
    • 1
  • E. Schleicher
    • 1
  • H. Simon
    • 1
  1. 1.Technical University MunichMunichFederal Republic Germany

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