Immobilization of 3β-Hydroxysteroid Dehydrogenase Isolated from Streptomyces griseocarneus
A Streptomyces griseocarneus strain was selected from 600 actinomyces tested to transform 3β,l7α,2l --trihydroxy-5-pregnen-20-one-3,21-diacetate into 17α,21--dihydroxy-4-pregnen-3,20-dione (compound Reichstein-S). This strain gave not only the highest yield of compound Reichstein-S, but the steroid transforming activity was not reduced in the presence of water immiscible organic solvents. This biosynthetic method, designed to obtain compound Reichstein-S by nonproliferating bacterium cells, has been patented (1).
KeywordsCorn Steep Liquor Organic Nitrogen Source Inorganic Nitrogen Source Hydroxysteroid Dehydrogenase Soya Extract
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