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Isocyanide Derivatives of Polysaccharides as Supports for Enzyme Immobilization

  • A. Freeman
  • M. Sokolovsky
  • L. Goldstein

Abstract

Polymers containing isocyanide functional groups offer versatile supports for the covalent fixation of proteins and other biologically active molecules via four-component condensation reactions involving amine, carboxyl, aldehyde and isocyanide, (1–6) as follows:
$$ {R^1} - COOH + {R^2} - N{H_2} + {R^3}CHO + CH - {R^4} \to {R^1}CON\left( {{R^2}} \right)CH\left( {{R^3}} \right)CONH{R^4} $$
In this reaction, carried out at neutral pH, the polymeric support supplies the isocyanide component, and the protein supplies either the amine or the carboxyl components. A water-soluble aldehyde, e.g. acetaldehyde, and the missing fourth component are added to the aqueous medium. The isocyanide functional groups can also be transformed into many other types of chemically reactive species which could be employed for covalent binding of proteins (2–4).

Keywords

Enzyme Immobilization Covalent Binding Polymeric Support Cellulose Powder Cyanogen Bromide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    GOLDSTEIN, L., FREEMAN, A. & SOKOLOVSKY, M. in “Enzyme Engineering,” vol. 2 (E.K. Pye. and L.B. Wingard eds.), Plenum Press, New York, 1974, p. 97.Google Scholar
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    GOLDSTEIN, L., FREEMAN, A. & SOKOLOVSKY, M. Biochem. J. 143: 497, 1974.Google Scholar
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    FREEMAN, A., SOKOLOVSKY, M. & GOLDSTEIN, L. J. Solid-Phase Biochem. 1: 261, 1977.Google Scholar
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    FREEMAN, A., GRANOT, R., SOKOLOVSKY, M. & GOLDSTEIN, L. J. Solid-Phase Biochem. 1: 275, 1977.Google Scholar
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    GOLDSTEIN, L., FREEMAN, A., BLASSBERGER, D., GRANOT, R. & SOKOLOVSKY, M. in “Proceedings Symposium on Biotechnological Application of Proteins and Enzymes,” (Z. Bohak and N. Sharon, eds.), Academic Press, New York, in press.Google Scholar
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    BLASSBERGER, D., FREEMAN, A. & GOLDSTEIN, L. Biotech-not. Bioeng.,in press.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • A. Freeman
    • 1
  • M. Sokolovsky
    • 1
  • L. Goldstein
    • 1
  1. 1.Department of BiochemistryTel Aviv UniversityTel AvivIsrael

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