Staphylococcal Nuclease Immobilized on Agarose Gel

  • Jose M. Guisan
  • Antonio Ballesteros


The enzyme micrococcal nuclease (ribonucleate (deoxyribonucleate) 3’-nucleotidohydrolase, EC. from Staphylococcus aureus has been extensively studied by Anfinsen and collaborators (1). They have elucidated the primary structure, the catalytic mechanism and details of the active centre. The X-ray structure of this enzyme at 2Å resolution is also known (2). We report here the immobilization on different supports and the analysis of the properties of the immobilized enzyme.


Immobilize Enzyme Catalytic Efficiency Soluble Enzyme Nuclease Activity Staphylococcal Nuclease 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    ANFINSEN, C.B., CUATRECASAS, P. & TANIUCHI, H. in “The Enzymes,” 3rd Edit., vol. 4 (P.D. Boyer, ed.), Academic Press, New York, 1971, p. 177.Google Scholar
  2. 2.
    COTTON, F.A. & HAZEN, E.E. JR. in “The Enzymes,” 3rd Edit., vol. 4, (P.D. Boyer, ed.), Academic Press, New York, 1971, p. 153.Google Scholar
  3. 3.
    CUATRECASAS, P., FUCHS, S. & ANFINSEN, C.B. J. Biol. Chem. 242: 1541, 1967.Google Scholar
  4. 4.
    CUATRECASAS, P., WILCHEK, M. & ANFINSEN, C.B. Biochemistry 8: 2277, 1969.CrossRefGoogle Scholar
  5. 5.
    REGAN, D.L., LILLY, M.D. & DUNNILL, P. Biotechnol. Bioeng. 16: 1081, 1974.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Jose M. Guisan
    • 1
  • Antonio Ballesteros
    • 1
  1. 1.Consejo Superior de Investigaciones CientificasInstituto de Catalisis y PetroleoquimicaMadridSpain

Personalised recommendations