Staphylococcal Nuclease Immobilized on Agarose Gel
The enzyme micrococcal nuclease (ribonucleate (deoxyribonucleate) 3’-nucleotidohydrolase, EC.188.8.131.52) from Staphylococcus aureus has been extensively studied by Anfinsen and collaborators (1). They have elucidated the primary structure, the catalytic mechanism and details of the active centre. The X-ray structure of this enzyme at 2Å resolution is also known (2). We report here the immobilization on different supports and the analysis of the properties of the immobilized enzyme.
KeywordsImmobilize Enzyme Catalytic Efficiency Soluble Enzyme Nuclease Activity Staphylococcal Nuclease
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