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Immobilization of Acetate Kinase on Functionalized Solid-Core Polymeric Beads

  • B. A. Solomon
  • C. C. Chen
  • C. K. Colton

Abstract

We present here a method for immobilizing enzymes on a new support material useful in various reactor configurations: very small (1 to 3 pm diameter), high specific surface area (6 m2/g), solid acrylate-based polymeric beads having carboxylic acid moieties (0.4 meq/g) on the surface (Matrex 101, Amicon Corp.). Acetate kinase (AcK) was selected because of its potential use in regeneration of adenosine triphosphate (ATP) (1)

Keywords

Borate Buffer Aldehyde Group Adenosine Triphosphate Polymeric Bead Acetate Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    LANGER, R.S., HAMILTION, M.C. & COLTON, C.K. AIChE J. 22:1079, 1976. Google Scholar
  2. 2.
    LANGER, R. S . , GARDNER, C C.K. AIChE J. 23:1, 1977.Google Scholar
  3. 3.
    NEMET, M., Ph.D. Thesis, MIT, Cambridge, 1976.Google Scholar
  4. 4.
    CHEN, C.-C., M.S. Thesis, MIT, Cambridge, 1976.Google Scholar
  5. 5.
    DATTA, R., ARMIGER, W. & Ollis, D.F. Biotechnoe Bioeng. 15: 993, 1973.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • B. A. Solomon
    • 1
  • C. C. Chen
    • 1
  • C. K. Colton
    • 1
  1. 1.Department of Chemical Engineering Massachusetts Institute of TechnologyCambridgeUSA

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