Membrane Reactor-Separator for Continuous Enzymatic Regeneration of ATP

  • Colin R. Gardner


A potentially useful application of cell-free enzyme systems is the production of biologically important compounds (1,2). Biosynthetic reactions require the input of chemical energy usually in the form of ATP; but a continuous supply of ATP at current prices would make such reactions prohibitively expensive. During the reaction ATP is degraded to ADP or AMP. We have shown previously (2-4) that it is economically feasible to use cell-free enzymatic systems to regenerate ATP from ADP or AMP using adenylate kinase to convert AMP plus ATP to ADP and acetate kinase to react ADP and acetylphosphate to form ATP and acetate. We have already demonstrated several methods by which the acetate might be removed (3).


Composite Membrane Adenylate Kinase Ultrathin Film Current Prex Acetate Kinase 


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  1. 1.
    HAMILTON, B.K., MONTGOMERY, J.P., & WANG, D.I.C. in “Enzyme Engineering” vol. 2, (E.K. Pye and L.B. Wingard, Jr., eds.), Plenum Press, New York, 1974, p. 153.Google Scholar
  2. 2.
    WHITESIDES, G.M. in “Applications of Biochemical Systems in Organic Chemistry,” (J.B. Jones, C.J. Sih and D. Perlman, eds.), Wiley, New York, 1976, p. 901 and 917.Google Scholar
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    GARDNER, C.R., COLTON, C.K., LANGER, R.S., HAMILTON, B.K., ARCHER, M.C. & WHITESIDES, G.M. in “Enzyme Engineering” vol. 2 (E.K. Pye and L.B. Wingard, Jr., eds.), Plenum Press, New York, 1974, p. 209.Google Scholar
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    MORRIS, D.L., CAMPBELL, J., & HORNBY, W.E. B.Lachem. J. 147: 593, 1975.Google Scholar

Copyright information

© Plenum Press, New York 1978

Authors and Affiliations

  • Colin R. Gardner
    • 1
  1. 1.Centre de Recherche MerrellInternational StrasbourgFrance

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