Affinity Chromatography of Kinases and Dehydrogenases on Sephadex® and Sepharose® Dye Derivatives
Biospecific affinity chromatography has been shown to be a powerful method for the isolation of biological compounds1,2. While high specificity of the immobilized ligand for the compound of interest generally results in high purification factors and simple isolation protocols, it usually necessitates the often laborious synthesis of a special affinity adsorbent. Affinity gels with group rather than compound specificity therefore have the potential advantage of being useful for the isolation of many compounds belonging to a particular group. The resolving po!’.er of these gels need not be less than the more compound specific gels, since highly selective compound elution is still possible with substrates, cofactors, or inhibitors.
KeywordsCreatine Kinase Pyruvate Kinase Blue Dextran Cibacron Blue Cibacron Blue F3GA
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