Cooperative Effects of AMP, ATP and Fructose 1,6-P2 on the Specific Elution of Fructose 1,6-Diphosphatase from Cellulose Phosphate
In 1963 Mendicino and Vasarhely (1) first observed that fructose 1,6-diphosphatases from kidney and liver were specifically bound to cellulose phosphate and carboxymethylcellulose. These studies further clearly demonstrated that AMP and high concentrations of fructose 1,6-P2 inhibited the activity of the enzyme (1). Since this time a number of other workers have utilized the specific binding of this enzyme to anion exchange columns as the principal purification step in the isolation of the enzyme from a large number of different tissues (2,3,4,5).
KeywordsCationic Group Cooperative Effect Phosphoryl Group Anion Exchange Column Sulfhydryl Reagent
Unable to display preview. Download preview PDF.
- 1.Mendicino, J. and Vasarhely, F. (1963) J. Biol. Chem., 240, 3528.Google Scholar
- 6.Mendicino, J., Prihar, H. S. and Salama, F. M. (1968) J. Biol. Chem., 243, 2710–2717.Google Scholar
- 7.Kratowich, N. and Mendicino, J. (1970) J. Biol. Chem., 245, 2483–2492.Google Scholar
- 8.Mendicino, J., Kratowich, N. and Oliver, R. M. (1972) J. Biol. Chem., 247, 6643–6650.Google Scholar
- 9.Giovanna, C., Hubert, E. and Marcus, E. (1972) Biochemistry, 11, 1796–1801.Google Scholar
- 10.Exton, J. H. and Park, C. R. (1969) J. Biol. Chem., 244, 1424–1431.Google Scholar