Cooperative Effects of AMP, ATP and Fructose 1,6-P2 on the Specific Elution of Fructose 1,6-Diphosphatase from Cellulose Phosphate

  • Joseph Mendicino
  • Hussein Abou-Issa
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)


In 1963 Mendicino and Vasarhely (1) first observed that fructose 1,6-diphosphatases from kidney and liver were specifically bound to cellulose phosphate and carboxymethylcellulose. These studies further clearly demonstrated that AMP and high concentrations of fructose 1,6-P2 inhibited the activity of the enzyme (1). Since this time a number of other workers have utilized the specific binding of this enzyme to anion exchange columns as the principal purification step in the isolation of the enzyme from a large number of different tissues (2,3,4,5).


Cationic Group Cooperative Effect Phosphoryl Group Anion Exchange Column Sulfhydryl Reagent 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Joseph Mendicino
    • 1
  • Hussein Abou-Issa
    • 1
  1. 1.Department of BiochemistryUniversity of GeorgiaAthensUSA

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