Purification of Acetylcholinesterase by Covalent Affinity Chromatography
Although the concept of affinity chromatography for the purification of macromolecules goes back many years, it has been only in the last five years or so that the field has blossomed. This development has been fostered by the creation and availability of numerous types of gels for chromatography and by the efforts of a number of highly skillful and imaginative researchers.(1) The standard type of affinity chromatography involves ligands that reversibly bind to the protein with a high degree of specificity. If the protein is an enzyme these ligands are usually selected from reversible inhibitors.
KeywordsAffinity Chromatography Sucrose Gradient Electric Organ Leaving Group 2PAM Solution
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