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Purification of Acetylcholinesterase by Covalent Affinity Chromatography

  • Houston F. Voss
  • Y. Ashani
  • Irwin B. Wilson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Abstract

Although the concept of affinity chromatography for the purification of macromolecules goes back many years, it has been only in the last five years or so that the field has blossomed. This development has been fostered by the creation and availability of numerous types of gels for chromatography and by the efforts of a number of highly skillful and imaginative researchers.(1) The standard type of affinity chromatography involves ligands that reversibly bind to the protein with a high degree of specificity. If the protein is an enzyme these ligands are usually selected from reversible inhibitors.

Keywords

Affinity Chromatography Sucrose Gradient Electric Organ Leaving Group 2PAM Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Houston F. Voss
    • 1
  • Y. Ashani
    • 2
  • Irwin B. Wilson
    • 1
  1. 1.Department of ChemistryUniversity of ColoradoBoulderUSA
  2. 2.Israel Institute for Biological ResearchNess-ZionaIsrael

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