A Solid Phase Radioimmune Assay for Ornithine Transcarbamylase

  • Donald L. Eshenbaugh
  • Donald Sens
  • Eric James
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)


Radioimmune assays utilizing solid supports were introduced by Catt et al (1966) and Wide and Porath (1966). The classical radioimmune assay described by Yalow and Berson (1960) is based on the ability of unlabeled antigen to compete with antigen labeled with a radioactive isotope bound to antibody. After permitting the system to reach equilibrium antibody bound radioactivity was separated from unbound radioactivity and one or the other determined in order to ascertain the quantity of unlabeled antigen in the unknown sample.


Ammonium Sulfate Cyanogen Bromide Ornithine Transcarbamylase Carbamyl Phosphate Sodium Thiocyanate 
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Donald L. Eshenbaugh
    • 1
  • Donald Sens
    • 1
  • Eric James
    • 1
  1. 1.Department of ChemistryUniversity of South CarolinaColumbiaUSA

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