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Quantitative Parameters in Affinity Chromatography

  • A. H. Nishikawa
  • P. Bailon
  • A. H. Ramel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Abstract

While the notion of purifying biopolymers in general (and proteins in particular) by selectively adsorbing them onto a specific carrier was reported some time ago (Lerman, 1953), a great surge in the application of this technique has recently followed the introduction of the term “affinity chromatography” (Cuatrecasas, et al. 1968). While mnemonically useful, this term does not adequately describe many of the processes reported in papers on affinity chromatography. Most papers typically describe a solidphase adsorption process using a specifically designed carrier material. Rarely is any description made of relative mobilities, of partition coefficients, or of separation factors. Indeed a column is not even required as demonstrated by Porath (1972) and others.

Keywords

Affinity Chromatography Ligand Concentration Cyanogen Bromide High Ligand Equilibrium Binding Study 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Cuatrecasas, P., Wilchek, M., and Anfinsen, C.B. (1968) Proc. Nat. Acad. Sci., USA 61, 636.Google Scholar
  2. Hixson, H.F., and Nishikawa, A. H. (1973) Arch. Biochem. Biophys. 154, 501.Google Scholar
  3. Lerman, L. (1953) Proc. Nat. Acad. Sci., USA 39, 233.Google Scholar
  4. Porath, J., and Sundberg, L. (1972) in “The Chemistry of Biosur-faces”, Edit. by M.L. Hair, Marcel Dekker, N.Y.C., Chap. 14.Google Scholar
  5. Robert-Gero, M., and Waller, J. P. (1972) Eur. J. Biochem. 31, 315.CrossRefGoogle Scholar
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Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • A. H. Nishikawa
    • 1
  • P. Bailon
    • 1
  • A. H. Ramel
    • 1
  1. 1.Chemical Research DivisionHoffmann-La Roche Inc.NutleyUSA

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