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Immobilization of Lipase to Cyanogen Bromide Activated Polysaccharide Carriers

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Immobilized Biochemicals and Affinity Chromatography

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 42))

Abstract

Brandenberger attached lipase to the isocyanato derivative of poly-p-aminostyrene in 1956 (1). This was the first time that lipase was immobilized to an insoluble carrier through covalent bond formation. Kitajima also entrapped the lipase in microcapsule (2). The present paper describes the preparation and some properties of lipase bound to polysaccharides following cyanogen bromide activation.

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References

  1. H. Brandenberger, Rev. Ferment. Ind. Aliment., 11, 237, 1956.

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  2. M. Kitajima, S. Miyano, and A. Kondo, Kogyo Kagaku Zasshi, 72, 493, 1969.

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  3. P. Melius, and W.S. Simmons, Biochim. Biophys. Acta. 105, 600–602, 1965.

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© 1974 Plenum Press, New York

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Melius, P., Wang, BC. (1974). Immobilization of Lipase to Cyanogen Bromide Activated Polysaccharide Carriers. In: Dunlap, R.B. (eds) Immobilized Biochemicals and Affinity Chromatography. Advances in Experimental Medicine and Biology, vol 42. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-6982-0_26

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  • DOI: https://doi.org/10.1007/978-1-4684-6982-0_26

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-6984-4

  • Online ISBN: 978-1-4684-6982-0

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