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Immobilization of Lipase to Cyanogen Bromide Activated Polysaccharide Carriers

  • Paul Melius
  • Bi-Chong Wang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)

Abstract

Brandenberger attached lipase to the isocyanato derivative of poly-p-aminostyrene in 1956 (1). This was the first time that lipase was immobilized to an insoluble carrier through covalent bond formation. Kitajima also entrapped the lipase in microcapsule (2). The present paper describes the preparation and some properties of lipase bound to polysaccharides following cyanogen bromide activation.

Keywords

Coupling Yield Immobilize Lipase Cyanogen Bromide Porcine Pancreatic Lipase Covalent Bond Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    H. Brandenberger, Rev. Ferment. Ind. Aliment., 11, 237, 1956.Google Scholar
  2. 2.
    M. Kitajima, S. Miyano, and A. Kondo, Kogyo Kagaku Zasshi, 72, 493, 1969.CrossRefGoogle Scholar
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    P. Melius, and W.S. Simmons, Biochim. Biophys. Acta. 105, 600–602, 1965.Google Scholar
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    R. Axen, and S. Ernback, Eur. J. Biochem., 18, 351, 1971.CrossRefGoogle Scholar
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    J. Fritz, and P. Melius, Canad. J. Biochem. Physiol., 41, 719 1963.CrossRefGoogle Scholar
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    E.M. Crook, K. Brocklehurst, and C.W. Wharton, Methods in Enzymology, 19, 963, 1970.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • Paul Melius
    • 1
  • Bi-Chong Wang
    • 1
  1. 1.Department of ChemistryAuburn UniversityAuburnUSA

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