Chemical Modification of Mushroom Tyrosinase for Stabilization to Reaction Inactivation
The utilization of chemical modification to evoke or favor a particular response while avoiding an undesirable characteristic of the protein is nothing new. Formaldehyde has been used to modify bacterial toxins, rendering them incapable of eliciting a toxic response, but still able to produce an immunological response when injected into an animal. The ancient process of tanning has been improved by the use of glutaraldehyde, a cross-linking agent. Insolubilization of enzymes for use in column or filtration techniques for the destruction or alteration of substances has received much recent attention. Hence, immobilization and subsequent modification of an enzyme, or immobilization of the modified enzyme, would seem to be a reasonable procedure.
KeywordsImmobilize Enzyme Flow Reactor Free Enzyme Dopa Content Collagen Membrane
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