Analysis of Reactions Catalyzed by Polysaccharide-Enzyme Derivatives in Packed Beds
The reaction catalyzed by an insoluble enzyme derivative in a packed reactor bed is typically analyzed by the integrated Michaelis-Menten equation (1,2,3,4). The value of KM is found to vary with the flow rate of the substrate solution through the bed. Lilly, Hornby and Crook (1) suggest that this phenomenon is due to the effect of diffusion limitations on the rate of reaction in the case of ficin insolublized to CM-cellulose.
KeywordsCyanogen Bromide Batch Method Initial Substrate Concentration Cyclic Monophosphate Column Method
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