Analysis of Reactions Catalyzed by Polysaccharide-Enzyme Derivatives in Packed Beds

  • M. H. Keyes
  • F. E. Semersky
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 42)


The reaction catalyzed by an insoluble enzyme derivative in a packed reactor bed is typically analyzed by the integrated Michaelis-Menten equation (1,2,3,4). The value of KM is found to vary with the flow rate of the substrate solution through the bed. Lilly, Hornby and Crook (1) suggest that this phenomenon is due to the effect of diffusion limitations on the rate of reaction in the case of ficin insolublized to CM-cellulose.


Cyanogen Bromide Batch Method Initial Substrate Concentration Cyclic Monophosphate Column Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Lilly, M. D., Hornby, W. E., and Crook, W. M., Biochem. J. 100, 718 (1966).Google Scholar
  2. 2.
    Sharp, A. K., Kay, G., and Lilly, M. D., Biotechnology and Bioengineering 11, 363 (1969).CrossRefGoogle Scholar
  3. 3.
    Lilly, M. D., and Sharp, A. K., Institution of Chem. Eng. Trans. 215, 12 (1968).Google Scholar
  4. 4.
    Bar-Eli, A., and Katchalski, E., J. Biol. Chem. 238, 1690 (1963).Google Scholar
  5. 5.
    Yapel, A., Han, M., Lumry, R., Rosenberg, A., and Shiao, D. F., J. Am. Chem. Soc. 88, 2573 (1966).CrossRefGoogle Scholar
  6. 6.
    Hammes, G. G., and Walz, F. G. Jr., Biochim. Biophys. Acta 198, 604 (1970).Google Scholar
  7. 7.
    Baum, H., Hübscher, G., and Mahler, H. R., Biochim. Biophys. 22, 514 (1956).CrossRefGoogle Scholar
  8. 8.
    Hummel, B. C. W., Can. J. Biochem. Physiol. 37, 1393 (1959).CrossRefGoogle Scholar
  9. 9.
    Walter, C., in Steady-State Applications in Enzyme Kinetics, p. 34. The Ronald Press Company, New York, 1965.Google Scholar
  10. 10.
    Sundaram, P. V., Tweedale, A., and Laidler, K. J., Can. J. Chem. 48, 1498 (1970).CrossRefGoogle Scholar
  11. 11.
    Cleland, W. W., Adv. in Enzymology 29, 1 (1967).Google Scholar
  12. 12.
    Herries, D. G., Mathias, A. P., and Rabin, B. R., Biochem.J. 85, 127 (1962).Google Scholar
  13. 13.
    Kistinkowsky, G. B., and Shaw, W. H. R., J. Am. Chem. Soc. 75, 2751 (1953).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1974

Authors and Affiliations

  • M. H. Keyes
    • 1
  • F. E. Semersky
    • 1
  1. 1.Owens-Illinois Technical CenterToledoSpain

Personalised recommendations