Water Encapsulated Enzymes in an Oil-Continuous Reactor-Kinetics and Reactivity
The organism used in this study was a species of Pichia yeast. Media and culture conditions have been described previously (1). Cultures were routinely grown in the presence of 3 weight percent of n-hexadecane as the sole source of carbon. Washed cell suspensions prepared for sonic extraction consisted of approximately 27 grams of wet cells (25% dry weight), suspended in 80 ml. of pH 8.0 phosphate buffer. Sonic extracted enzyme solutions were separated from cell fragments by centrifugation for 15 minutes at 34, 000 × g. at 0 °C. Generally, the enzyme solutions contained 10–12 mg/ml. of protein.
KeywordsInduction Period Continuous Flow Reactor Product Formation Rate Aldehyde Formation Sonic Extraction
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